Background This study aimed to compare the shaping ability of OneShape, Hero Shaper, and Revo-S instruments in simulated L-shaped canals. Methods forty-eight simulated L-shaped canals were prepared to an apical size of 25 using OneShape, Hero Shaper, and Revo-S (all from Micro-Mega SA, Besançon, France), (n = 16 canals/group) systems. The amount of resin removed after each canal's preparation was measured and compared after producing a composite image made from the superimposition of pre and post-instrumented canals. Canal aberrations and the preparation time were also recorded. The data were statistically analyzed by using ANOVA, Tukey, and Chi-square tests. Results One file fractured during instrumentation in Revo-S group. A significant difference was found at the apical end of the prepared simulated canal between the groups, with Revo-S showing the least amount of resin removal from the inner side of the canals and Hero Shaper showing the highest amount of resin removal from the outer side (P < 0.05). Regarding the total width of the canals after preparation, a significant difference was found between the groups at the apical end and the straight portion of the canals, and Revo-S removed the least amount of resin at the straight portion of the canals (P < 0.05). No statistically significant differences were found between the different instruments regarding canal aberrations' incidence (P > 0.05). Conclusions all of the files showed a tendency to straighten the canals, whereas OneShape files maintained the original canal curvatures well.