An effort was made to synthesize a new series of pyrazolo [3,4-d] pyrimidine derivatives (2-5). The synthesis of these compounds was achieved via reaction of 3-methyl-1,4-dihydropyrazol-5-one (1) with a substituted aromatic aldehyde and urea or thiourea in mild reaction conditions, giving satisfactory yields. FT-IR and 1 H-NMR spectroscopies were used to characterize the structure of the newly synthesized compounds (2-5), where the spectral data confirmed the formation of these compounds. The antioxidant activity of these compounds (2-5) was examined in this study the synthesized compounds showed a moderate-high antioxidant effect, which was examined by using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assays. The cellular toxicity of the compounds (2-5) was studied on MCF-7 cell lines using MTT assay. Compound (5) showed the highest toxicitytowards MCF-7 cell lines.

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