Ocimum basilicum has been widely used in traditional medicine. Rural communities have used fixed oils for variety purposes since a long time ago. They used for cosmetic applications, fuel, medicine and food. The aim of this study was to characterize the physicochemical properties and fatty acid composition of O. basilicum seed oil. Lipids were determined by continuous extraction in a Soxhlet apparatus for 6 hours using hexane as solvent. The physicochemical properties of the oil were assessed by standard and established methods. The fatty acids composition of the seed oil was determined by GC-MS. The Pale yellow with camphor odor oil extracted from the seed has the following properties: yield, 18.01%; freezing point, -2°C; melting point, 5°C; boiling point, 215°C; refractive index (25°C), 1.48532; iodine value, 108.6 g/100 g of oil; peroxide value, 4.6 meq. O2/kg of oil; free fatty acids, 0.20%; acid value, 4.0 mg of KOH/g of oil; saponification value, 164.2 mg KOH/g of oil; unsaponifiable matter, 1.6; moisture and volatile value, 4.97 (wt%); density, 0.91372 g/cm3; viscosity, 10.29 mm2/s; specific gravity, 0.9210. Fatty acids composition showed that linolenic- (43.92%) was the major fatty acid and followed by linoleic- (32.18%), palmitic- (13.38%), stearic- (6.55%), palmitoleic- (0.78%), arachidic- (0.72%), anteisomargaric- (0.45%), nonadecylic- (0.28%), gondoic- (0.27%), margaric- (0.20%), behenic- (0.17%), heneicosylic- (0.14%), lignoceric- (0.13%) and myristic acid (0.11%). Therefore, recommended that more and advanced investigations should be undertaken for this abundant oil as natural source for many industrial applications, especially, for applications that require acids like linolenic and linoleic.
Moringa oleifera is a robust and fast-growing tree considered as one of the most beneficial trees worldwide since almost all parts of it are used as food, medicine, and for industrial purposes. This study aimed to investigate the physicochemical properties and fatty acid composition of M. oleifera seed oil. The oil was extracted by Soxhlet using n-hexane; the physicochemical properties of the seed oil were assessed by standard and established methods, as well, the fatty acid composition of the seed oil was determined by GC-MS. The golden yellow oil with characteristic odor obtained from the seeds had the following physicochemical properties: yield, 42.87%; freezing point, 0 °C; melting point, 21 °C; boiling point, 225 °C; refractive index (25 °C), 1.447; iodine value, 96.6 g/100g of oil; peroxide value, 7.6 meq.O2/kg of oil; free fatty acids, 0.07%; acid value, 1.4 mg of KOH/g of oil; saponification value, 185.2 mg KOH/g of oil; unsaponifiable matter, 3.2; moisture and volatile value, 4.91 (wt.%); density, 0.900 g/cm 3 ; viscosity, 60.99 mm 2 /s; specific gravity, 0.907. The fatty acids composition showed that oleicacid (51.74%) was the major fatty acid and followed by behenic-(10.54%), palmitic-(9.20%), stearic-(8.46%), arachidic-(6.41%), gondic-(4.88%), lignoceric-(3.08%) and palmitoleic acid (2.85%). Therefore, more and advanced research should be undertaken for this abundant source of natural oil for edible oil and possible industrial applications.
Basil (Ocimum basilicum L.) is an important culinary herb and essential oil source widely recognized worldwide. The oil of the plant is beneficial for medicinal uses, and it has many biological activities such as insect repellent, larvicidal, and bactericidal. This study aimed to investigate basil's essential oil for its chemical composition and repellent activity. The essential oil of basil was extracted from fresh leaves by steam distillation method, and the chemical composition of the oil was determined by using GC/MS. Also, the repellent activity of the oil was tested against American cockroaches. Ebeling Choice-Box test with a little modification used in repellence test. The obtained results of chemical composition revealed that the amount of the oil contained forty-one chemical constituents (~97.1%); the major constituents were methyl cinnamate (25.3%), linalool (19.1%) and estragole (12.3%) as the major oxygenated monoterpenes. While α-bergamoten (5.3%), germacrene (4.6%), γ-cadinene (2.8%), and β-elemene (2.4%) were the main compounds in sesquiterpene hydrocarbons. Whereas, in oxygenated sesquiterpenes, Tau-cadinol (4.3%) was an important compounds and ocimene the highest compound in monoterpene hydrocarbons. The obtained results also indicated that the essential oil had good activity against the P. American, at a 100% concentration of oil; the repellence reaches 100% after 1 h. The IC50 and IC90 values of basil essential oil against P. Americans were 53.0 and 83.0%, respectively. The major compound methyl cinnamate which exists in basil essential oil is a very important compound and could be used in a wide area of industrial applications as repellent products, medicinal products, and cosmetics.
Cockroaches are among the most adaptive creatures to the environment; they dwell on the earth's surface for the past millions of years. The presence of cockroaches is posing serious health issues in many countries of the world. Searching for an effective natural repellent agent has become a highly important attempt to control these insects. Hence, this study aimed to evaluate the chemical compositions of essential oil from Sudanese Cyperus rotundus rhizomes and determine their repellent activity against American cockroaches (Periplaneta americana). The obtained essential oil was radish yellow in color, the oil content was 0.73% and forty-four constituents were identified. The most major components were: Isolongifolol (7.63%), longiverbenone (5.61%), β-cadinene (5.54%), and longifolenaldehyde (5.16%). The oil was characterized by sesquiterpenes abundance 52.45% (oxygenated sesquiterpenes 30.72% and sesquiterpenes hydrocarbons 21.73%); whereas, monoterpenes comprised 8.43% (oxygenated monoterpenes 6.81%, and monoterpenes hydrocarbons 1.62%). The experiment showed that the essential oil possesses promising results as a repellent against P. americana. Therefore, conducting more research on isolation, identification of active compounds and extensive tests on other types of cockroaches are priorities in future studies.
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