Background: Attitude is very important concept in nursing due to high ambiguities and role conflicts within that profession. The globally widespread outbreak of COVID-19 pandemic all over the world affected all races and socioeconomic classes with its unprecedented negative effect on the usual way of living. This survey study aimed to identify junior nursing students' attitude toward the profession during COVID-19 pandemic. Methods: Utilizing a quantitative cross-sectional design through an electronic survey form developed by the investigators in two parts on personal profile items and the attitude toward nursing profession scale was carried out for collecting data using total enumeration as sampling technique; all students enrolled in the 1 st and 2 nd academic levels at the Faculty of Nursing -Minia University during the Academic year 2020/2021 (n= 1406) were involved in the survey with voluntary and anonymous participation. Results: The study findings revealed that most of the students had a positive attitude toward nursing profession (97.3%) while only (2.7%) had a negative attitude with total mean score of the attitude level was 161.3 ± 19.2. Significant differences in overall attitude of the participated students in relation to their personal profile were favored to females, rural resided and second academic level students. Conclusion: The attitude of the junior nursing students during COVID-19 pandemic appeared to be positive towards their future profession. Against the investigators' expectations, findings indicated that 2 nd year students exhibited more positive attitude toward the nursing profession during COVID-19 pandemic than 1 st. year students.