Entandrophragma angolense plant was harvested at Dschang, Menoua Division (West Region, Cameroon) in June 2016. Plant parts such as stem bark were collected and authenticated by Mr Nana Victor, a botanist at the National Herbarium of Cameroon where a voucher specimen of the plant has been deposited under the number 29933/HNC. Experimental animal Eight weeks old female mice Balb/c weighing between 22 to 25 g were used for experimentation. ABSTRACT Background: Research for new antimalarial drugs remains a permanent quest for the control of malaria. Objective: The present study investigates the effects of the aqueous extract of Entandrophragma angolense bark on P. berghei-induced malaria in mice. Methods: Eight weeks old mice, were intraperitoneally infested with 200 μl of blood, containing 1x10 6 P. berghei-infected-erythrocytes. Parasitaemia was determined using a 10% giemsa stained blood smear read under optical microscope (x100). The infected animals were randomized into 5 groups of 10 animals each and daily treated for 5 days with the plant extract at 125, 250 and 500 mg/kg. The normal control and malaria control received water while the chloroquine control was treated with 10 mg/kg of chloroquine. Body weight, parasitaemia and survival time were monitored daily during treatment and follow up periods. Five animals from each group were sacrificed under anaesthesia at the end of treatment (d8) and after the follow up period (d28). Venous blood was used for haematological and biochemical tests. Organs (liver, kidneys and spleen) were also collected for biochemical and histological analyses. Results: Administration of the aqueous extract of E. angolense bark to infected mice significantly inhibited parasite development (p <0.001) with ED 50 estimated at 25.32 mg/kg. The extract prevented animal from death, body weight loss, anaemia, leucocytosis, high transaminases (ALT and AST), high bilirubin, creatinine and MDA levels, oxidative stress and anatomical alteration in organs as compared to the malaria control. Conclusion: The E. angolense bark possesses antimalarial properties, supporting its use in traditional medicine to treat malaria.

Background Research for new antimalarial drugs remains a permanent quest for the control of malaria disease due to the emerging parasite resistances. The present study investigates the effects of the aqueous extract of Entandrophragma angolense (E. angolense) bark on Plasmodium. berghei-induced malaria in mice. Methods Eight weeks old female mice, were intraperitoneally infested with 200 μL of mouse blood, containing 1x106 P. berghei-infected-erythrocytes. Parasitaemia was determined using a 10% giemsa stained blood smear read under optical microscope (x100). The infected animals were randomized into 5 groups of 10 animals each and daily treated for 5 days with the plant extract at 125, 250 and 500 mg / kg. The malaria control received distilled water (10 mL / kg) while the chloroquine control was treated with 10 mg / kg of chloroquine. A group of healthy mice was used as the normal control and received distilled water. Body weight, parasitaemia and survival time were monitored daily during treatment and follow up periods. Five animals from each group were sacrificed under anaesthesia at the end of treatment (d8) and after the follow up period (d28). Venous blood was used for haematological and biochemical tests. Organs (liver, kidneys and spleen) were also collected for biochemical and histological analyses. Results Administration of the aqueous extract of E. angolense bark to infected mice significantly inhibited parasite development (p < 0.001) with ED50 estimated at 25.32 mg / kg. The extract prevented animal from death, body weight loss, anaemia, leucocytosis, high transaminases (ALT and AST), high bilirubin, creatinine and MDA levels, oxidative stress and anatomical alteration in organs as compared to the malaria control. Conclusions The E. angolense bark possesses antimalarial properties, supporting its use in traditional medicine to treat malaria.

Introduction: In general medicine, male sexual dysfunction is a major source of worry and consultation. Finding alternative substances like plants to regulate these disturbances is motivated by the side effects of standard aphrodisiac medicines. In the current investigation, male rats were used to test the aphrodisiac effects of an aqueous extract of Hibiscus asper leaves. Methodology: Aphrodisiac effect of the extract was evaluated following a single daily administration of Hibiscus asper at doses of 50, 100 and 200 mg/kg respectively. Sexual behavioural parameters such as mounting and intromission frequencies, mounting, intromission and ejaculatory latencies, sexual motivation were monitored on days 1, 4 and 8. At the end of the experiment, the grapping test was assessed, after which the animals were sacrificed and blood collected for the evaluation of cholesterol, fructose, proteins, acid phosphatase, testosterone, and other androgen-dependent enzymes. Some androgen-dependent organs, such as the testis, epididymis, seminal vesicle, prostate, and levator ani muscle, were also removed for biochemical and histological analysis. Results: The administration of the aqueous extract of H. asper leaves had a significant impact on sexual behaviour, increasing mounting frequency (MF), intromission frequency (IF), and ejaculatory latency (EL), and reducing mounting latency (ML), intromission latency (IL), and post-ejaculatory interval (PEI). There was also an enhancement in orientational activities, libido, arousal and potency. Cholesterol, acid phosphatase, vesicular fructose, proteins, nitric oxide and testosterone levels were all significantly increased in treated animals. Conclusion: These findings support the traditional use of H. asper leaves to enhance male sexual behavior by demonstrating the aphrodisiac potential of these plants.