Tylophora indica (Burm. F.) Merr is an endangered plant which can be protected from extinction by its large scale production. Nodal segments of healthy plants are used as explants and cultured on MS Basal medium fortified with different growth regulators. Optimum shoot induction conditions from explants were established. In vitro and in vivo phytochemical test were done by using standard methods for chlorophyll, carbohydrates, proteins, lipids and starch. 3mg/l 2, 4 D showed maximum and success full callus production. Shoot initiation started in 7 days and best shoot regeneration reported with 3 mg/ml BAP in Basal medium. Combination of IBA and NAA in concentration 2 and 4 mg/l respectively proved to be best for root initiation. Concentration of chlorophyll, protein, lipid, carbohydrate, and starch in vitro and in vivo culture are investigated.

In vitro regeneration of memory enhancing plant Bacopa monneiri was observed by using nodal explant. In present study, shoot initiation was best seen in MS media in 3mg/l BAP concentration. Combination of BAP and IAA (1mg/l+3mg/l concentration) was best suited for root initiation. The rooted plantlets were hardened and successfully established in soil.

Fenugreek (Trigonella foenum-graecum) is an important spice; its dried seeds have wide application in food and beverages as a flavoring additive as well as in medicines. The objective of the present study was to evaluate the phytochemical constitution of ethanolic extract of dried plant parts (Leaf, Stem, Root and Seed) of Medicinal Herb Trigonella foenum-graecum. Qualitative and Quantitative analysis of phytochemical constituents' viz. Carbohydrate, Starch, Amino Acid, Protein, Lipid, Chlorophyll, Phenol and Alkaloid was performed by well-known test protocols available in the literature. Maximum amount of phytoconstituents was recorded in root part of plant. The results suggest that Trigonella foenum-graecum is a promising potential source of natural drug.