Sphingolipids and their metabolites are increasingly implicated in the pathogenesis of many metabolic and neurological diseases. It has been postulated that sphingolipids coalesce with cholesterol to form laterally segregated lipid domains that are involved in protein sorting and trafficking. In this work, we have explored the effect of metabolic depletion of sphingolipids on cell surface expression of the human serotonin 1A receptor, a neurotransmitter G protein-coupled receptor. We used fumonisin B 1 (FB 1 ), a fungal mycotoxin, to inhibit sphingolipid biosynthesis in HEK-293 cells stably expressing the human serotonin 1A receptor. Our results obtained using flow cytometric analysis and confocal microscopic imaging show that the cell surface population of the serotonin 1A receptor is reduced under sphingolipid-depleted condition. Western blot analysis confirmed that there was no significant difference in total cellular level of the serotonin 1A receptor upon depletion of sphingolipids. Interestingly, the effect of FB 1 on serotonin 1A receptor population was reversed upon replenishment with sphingolipids. These results indicate that sphingolipid depletion does not alter total cellular receptor levels, but impairs serotonin 1A receptor trafficking to the cellular plasma membrane. These results could provide mechanistic insights into the role of sphingolipids in modulation of neurotransmitter receptor signaling and trafficking in health and disease.

Ubiquitous conventional plastics, generally manufactured from finite, nonsustainable fossil fuels are non-biodegradable wonder entities but their ill effect on Mother Nature has subsequently raised major environmental concerns like their safe disposal, solid waste management and several potential hazards. Such concerns have fuelled initiatives for research globally towards development of sustainable and eco-friendly bioplastics. The new generation of plastics called 'bioplastics' are polymers of long chain of repeating monomer units that are classified as photodegradable, semi-biodegradable, chemically synthesized and polyhydroxyalkanoates (PHAs). The commonly emerged novel bioplastics are polyesters of hydroxyalkanoates (HAs) called PHAs, which are lipoidic storage materials found in the cytosol of vast and diverse forms of bacteria. Among 150 different PHAs known so far, poly- 3-hydroxybutyrate is the most common and comprehensively characterized PHA. Interestingly, PHAs are only completely biodegradable plastics with material properties comparable to conventional plastics that can be achieved by regulating the co-monomers incorporation into PHAs backbone. PHA bioplastics are exploited in the form of user-friendly goods viz. films, absorbable sutures, bone plates, drug carriers, etc. Besides advantages, such useful entity(s) has major shortcomings as well like high production cost compared to conventional plastics. Precisely, in PHAs production, about fifty percent of the overall price is due to the carbon substrates. Consequently, exploring novel cost-effective substrates is a major compulsion for successful commercialization of this bioplastic, which is anticipated to reduce the cost of production as a result of advancing and intensifying research work. This review presents an insight and patent developments in the field of PHAs bioplastics.

Purpose This SWOT (strengths, weaknesses, opportunities and threats) analysis was conducted to have a portrait of the competitiveness of Council of Scientific and Industrial Research (CSIR) on the global map. Being a 75-year-old scientific organization, CSIR has raised each and every laboratory with a specific mandate and competency. It is definite that the organization would get some assistance from these points, which are briefly stated, as the institutional data reside with the laboratory only. To some extent, they can be considered on their discretion; however, the organization has raised its position to international standards, but still it can consider few areas/suggestions from this analysis. These points are suggestive and flexible in nature, which are identified and defined to elevate this organization on the globe. Scientific publications do make a difference, but on the ground, if the technologies are not viable and received by public, it makes no difference. Today, India is fortunate to have a Prime Minister like Mr Modi, who always re-evaluates the issues and solutions so that the country moves from developing to a fast-pace developed nation. It is a fact that if these research organizations reorganize themselves and prioritize the research problems, they are capable enough to become a pioneer among the world R&D industry. This paper aims to analyze the organization and suggest few points with significant findings which can assist in scaling toward excellence on a global scale. Design/methodology/approach On the basis of random sampling, laboratories were identified where their achievements and contribution toward industry and society were evaluated. On the basis of their setup, the organization and laboratories were evaluated to frame-out a SWOT analysis. Findings SWOT analysis is a deliberate technique carried out to analyze the current situation of the system and to analyze the internal and external environment, which provides and edge to the organization to sustain. The major factors assessed were manpower, machines, money and funding, and achievements and deliverables were studied. Originality/value The research, i.e. SWOT, is unique in nature, as it was not carried out earlier on such a scale. To some extent, the points suggested in SWOT and conclusion can be considered on their discretion; however, the organization has raised its position to international standards, but still it can consider few areas/suggestions from this analysis. These points are suggestive and flexible in nature, which are identified and defined to elevate this organization on the globe. Scientific publications do make a difference, but on the ground; if the technologies are not viable and received by public, it makes no difference. Today, India is fortunate to have a Prime Minister like Mr Modi, who always re-evaluates the issues and solutions so that the country moves from developing to a fast-pace developed nation. It is a fact that if these research organizations reorganize themselves and prioritize the research problems, they are capable enough to become a pioneer among the world R&D industry.

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Scientific Productivity is a demand of policy makers for a judicious utilization of massive R&D budget allocated and utilized. A huge mass of intellectual assets is employed, which after investing manpower, infrastructure and lab consumables demand for a major outcome which contributes towards building nation's economy. Scientific productivity was only measured through publications or patents. Patents, earmarked as a strong parameter for innovation generation, where, Word Intellectual Property Organisation generated a data on applications for the top 20 offices for patents, where Australia, Brazil and Canada occupied top 3 positions. India ranked 9th with the total patent applications rising from 39762 (2010) to 42854 (2014) i.e. 15%, whereas, it contributes around 2% Patents (innovative productivity) on global scale. Many studies have come forward interestingly within scientific and academic domains in the form of measurement of scientific performance, however, development of productivity indicators and calculation of Scientific Productivity (SP) as a holistic evaluation system is a significant demand. SP, a herculean task is envisaged for productivity analysis and would submit significant factors towards fabricating an effective measurement engine in a holistic manner viable for an individual and organization, being supplementary to each other. This review projects the significance of performance measurement system in R&D through identification and standardization of key parameters. It also includes emphasis on inclusion of standardized parameters, effective for performance measurement which is applicable for scientists, technical staff as well as lab as a facility. This review aims at providing an insight to the evaluators, policy makers, and high level scientific panels to stimulate the scientific intellects on identified indicators so that their work proceeds to generate productive outcome contributing to the economic growth.