A survey of intestinal parasites of chickens was carried out in Akure, Ondo State, Nigeria from January to December, 2015. A total of 327 chickens of different breeds were examined for gastrointestinal infections. Fecal samples obtained from these chickens were prepared for microscopy using flotation technique. The results showed that 67 (20.5%) of the 327 chickens examined were infected with various gastrointestinal parasites. Among the infected chickens, the layers were the most susceptible to gastrointestinal parasites with a prevalence of 88.4%, while broilers were the least susceptible with a prevalence of 7.2%. It was further observed that the highest prevalence of gastrointestinal infection (37.6%) was recorded among the chickens that were kept in an extensive management system, while the lowest prevalence (9.6%) was recorded among the chickens kept in an intensive management system. Laboratory screening of the fecal samples for parasites revealed three types of protozoa: Eimeria spp. (7.7%), Histomonas meleagridis (0.6%), and Giardia lamblia (0.3%); and five types of nematodes: Ascaridia galli (7.0%), Heterakis gallinarum (1.8%), Capillaria spp. (0.9%), Syngamus trachea (0.6%), and Trichostrongylus tenuis (1.6%). Of all these parasites, Eimeria spp. was the most prevalent (7.7%), while Gardia lamblia was the least prevalent (0.3%). From the results of this study, it is evident that the chickens sold in the study area were infected with gastrointestinal parasites. Hence, it is suggested that an intensive poultry management system and regular treatment of the chickens should be encouraged in the study area.