The present study describes preparation and characterization of fibrin-chitosan-sodium alginate composite (F-C-SA) in sheet form. F-C-SA composite was prepared and characterized for its physicochemical properties like water absorption capacity, surface morphology, FTIR spectra and mechanical properties. The optimum quantities of fibrin, chitosan and sodium alginate to get better mechanical properties to composite were determined. FTIR spectrum confirmed the interaction between amino groups of chitosan, fibrin and sodium alginate and SEM studies revealed composite nature of the material.

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The organic response of polymer composites is facilitated by adding natural filler materials. Several new composite formulations claim to reduce the environmental impact. The present study assessed the cytotoxic responses of seashell and fish scale filled composite materials. The Polyethylene high-density (PEHD), 30 wt. % of the seashell and 30 wt. % of a seashell and fish scale combinations blended with the polymer matrix composites with 10:3 proportion (PEHD 100 g:Reinforcements 30 g), and are then fabricated by the plastic injection molding machine. To better imitator the microstructure and the mineral component of natural bone, novel hydroxyapatite / polymer composite scaffolds are allowed to interact 10, 20, 30, 40 and 50 μl of standard fresh cell culture medium for 24 hours. Morphology of cell, cell viability, and the effect of Cytotoxicity on polyethylene based composite samples were examined through ISO 10993:5, and 10993:12 test standards. The percentage of cell viability and the level of toxicity were compared.

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