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1.Affinity purified anti-renin was used to detect renin-immunoreactive proteins in cloned colonies of Escherichia coli containing recombinant mouse submandibular gland cDNA inserted at the PstI site of plasmid pBR322.2. Two colonies were detected which reacted positively with anti-renin.3. These colonies contained a single protein that could be immunoprecipitated with anti-renin and cDNA of more than 5 0 0 base-pairs in length which was capable of inter-colony hybridization in the single-stranded form and had similar Hinff restriction sites.4. Sequencing is being performed on the cDNA after having been subcloned in M13mp 7.1.

The serum concentration of zinc, copper, manganese and lead in 30 manic patients and 30 human volunteers were determined by flame atomic absorption spectroscopy. It was observed that the concentration of zinc in manic patients increased significantly (P < 0.05), where as the concentrations of copper, manganese and lead were found to be within the limit (P > 0.05). These concentrations of different trace elements may have some prognostic significance for the diagnosis of manic patients, but more work is suggested to find out the exact correlation between trace elements level & the degree of disorder in manic patients. Key words: Mania, trace elements, serum etc. Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

The aim of this study was to determine the serum immunoglobulin concentration in both manic patients and in healthy volunteers. The study was conducted with 30 manic patients (24 males and 6 females, aged 55.4 ± 13.0 years); age and gender-matched, control subjects were enrolled. An enzyme-linked immunosorbent assay (ELISA) was employed to analyze the serum immunoglobulin concentrations. IgG level was found to be 18.22±6.42 g/L in the manic patients, while it was 16.05 ± 3.45 g/l in the cohort controls (p=0.487). IgM and IgA concentrations were 6.025 ± 1.07 g/l and 1.41 ± 0.51 g/l in the manic patients, whereas those were 5.73 ± 1.63 g/l (p=0.688) and 0.837 ± 0.36 g/l (p=0.034) respectively in controls. From the results it was found that only the serum concentration of IgA was increased significantly (p=0.034) in manic patients while the concentrations of IgG and IgM remained unchanged. In many psychiatric disorders, immunoglobulin level has been found to be changed significantly. This study reveals that the concentration of IgA in manic patients increased significantly (p=0.034) which usually happens in case of many psychiatric patients. Further studies are suggested with larger population to find out the correlation between immunoglobulin level and degree of disorder and possibility of using as a diagnostic tool for the identification of this disease. Key words: Immunoglobulin, manic, psychiatry. Dhaka Univ. J. Pharm. Sci. Vol.4(2) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website