Berginia ligulata is medicinally significant and endangered plant of the western Himalayan Region. This plant is used to treat many diseases such as urinary problems and heart problems. By using leaf explant, an efficient and rapid micropropagation protocol was developed. In in-vitro propagation, MS medium was supplemented with benzyl amino purine (BAP), 1-naphthalene acetic acid (NAA) and indol-3-acetic acid (IAA) to induce multiple shoots from leaf explants. The most positive response for the formation of calluses was observed on MS media supplemented with MS medium + BAP(1 mg/L) + NAA(1 mg/L) followed by MS medium + BAP (0.5 mg/L) + NAA (1 mg/L) after 3 weeks. Maximum number of shoots were obtained on MS medium + BAP (2 mg/L) +IAA (2 mg/L). Maximum % shoot response was obtained on MS medium + Kn (2.5 mg/L) concentration. The maximum number of roots was observed on MS medium without growth regulators. These well-rooted plants were established in pots and acclimatized in the lab and transferred to field conditions. This process is useful to produce endangered plants on large scale.

The present study has developed a reproducible in vitro micropropagation protocol for endemic and endangered Thalictrum foliolosum by using leaf and root segments. Thalictrum foliolosum possesses high medicinal properties due to the presence of valuable phenols, flavonoids and alkaloids. The plants are uprooted due to their high medicinal and clinical applications, hence can lead to extinction threat. In vitro leaf and root, explant was used for induction of multiple shoots on MS medium supplemented with plant growth regulators like benzyl amino purine(BAP), indole-3- butyric acid (IBA), 1-Naphthalene acetic acid (NAA) and indole-3-acetic acid (IAA) alone or in combination.  The best positive response for the formation of shoots from the leaf segment was obtained from MS media supplemented with BAP (1.0 mg/l) + NAA (0.6 mg/l) after 4 weeks and Maximum roots were formed on MS + IBA (0.6 mg/l) within 4-5 weeks. Likewise, the maximum percentage of shooting and rooting from root explant was obtained in MS + BAP (0.8 mg/l) + TDZ (0.4 mg/l) and MS + IAA (1.0 mg/l) + NAA (2.0 mg/l) after 4 weeks. The well-rooted acclimatized plantlets were successfully transferred to field conditions. Plant Tissue Cult. & Biotech. 33(1): 47-56, 2023 (June)