Two new triterpenoid glycosides, together with two new ergostane glycosides, umbellatosides A -D (1 -4, resp.), have been isolated from the leaves of Hydrocotyle umbellata L. Their structures were established by 2D-NMR spectroscopic techniques ( 1 H, 1 H-COSY, TOCSY, NOESY, HSQC, and HMBC) and mass spectrometry as 3b,22b-dihydroxy and (3b,11a,21,26)-ergosta-5,24(28)-diene-3,11,21,26-tetrol Introduction. -The genus Hydrocotyle (Apiaceae) is constituted by aquatic or semiaquatic plants particularly well represented in temperate areas and higher tropical regions [1]. Some species were reported to have antioxidant and antitumoral properties [2] [3]. Triterpene saponins were isolated from several species of this genus [4 -7]. From H. umbellata, a herbaceous plant native to North America and parts of South America, this compound type has not been reported so far. In our continuing search for bioactive saponins from tropical plants, we report here the isolation and structure elucidation of two new triterpene saponins and two new ergostane glycosides, named umbellatosides A -D (1 -4, resp.), from the 70% MeOH extract of the leaves of H. umbellata.