Multicomponent groundwater tracer tests were conducted in a well‐characterized field site in Altona, NY using inert carbon‐cored nanoparticles and a thermally degrading phenolic compound. Experiments were conducted in a mesoscale reservoir consisting of a single subhorizontal bedding plane fracture located 7.6 m below ground surface contained between two wells separated by 14.1 m. The reservoir rock, initially at 11.7°C, was heated using 74°C water. During the heating process, a series of tracer tests using thermally degrading tracers were used to characterize the progressive in situ heating of the fracture. Fiber‐Optic Distributed Temperature Sensing (FODTS) was used to measure temperature rise orthogonal to the fracture surface at 10 locations. Recovery of the thermally degrading tracer's product was increased as the reservoir was progressively heated indicating that the advancement of the thermal front was proportional to the mass fraction of the thermally degrading tracer recovered. Both GPR imaging and FODTS measurements reveal that flow was reduced to a narrow channel which directly connected the two wells and led to rapid thermal breakthrough. Computational modeling of inert tracer and heat transport in a two‐dimensional discrete fracture demonstrate that subsurface characterization using inert tracers alone could not uniquely characterize the Altona field site. However, the inclusion of a thermally degrading tracer may permit accurate subsurface temperature monitoring. At the Altona field site, however, fluid‐rock interactions appear to have increased reaction rates relative to laboratory‐based measurements made in the absence of rock surfaces.

The spatial distribution of fracture/matrix heat exchange was measured while hot water was circulated through a single bedding plane fracture in a cold reservoir. Thermal breakthrough was recorded at the production well and Fiber-Optic Distributed Temperature Sensing (FODTS) monitored temperature in the rock matrix. Conservative tracer tests revealed that the reservoir fluid volume in two separate experiments were nearly identical. Thermal breakthrough measurements, however, revealed that reservoir fluid volume did not correlate to thermal performance because the two experiments encountered different heat transfer areas along the fracture. Ground Penetrating Radar imaging of subsurface tracer transport and FODTS corroborate these findings.

Field tests in a discrete rock fracture validated a combined inert/adsorbing tracer test method to estimate the contact area between fluids circulating through a fracture and the bulk rock matrix (i.e., flow‐wetted surface area, A). Tracer tests and heat injections occurred at a mesoscale well field in Altona, NY. A subhorizontal bedding plane fracture ∼7.6 m below ground surface connects two wells separated by 14.1 m. Recovery of the adsorbing tracer cesium was roughly 72% less than the inert tracer iodide. Using an advection‐dispersion‐reaction model in one‐dimension, the adsorbing/inert tracer method identified substantial flow channelization. These results are consistent with Ground Penetrating Radar (GPR) and thermal sensors. All characterization methods suggest circulating fluids were concentrated in a narrow, 1–2 m wide channel directly connecting the injection and production well. The inert/adsorbing tracer method identified two flow channels with areas of 28 and 80 m2. A one‐dimensional heat transport model predicted production well temperature rises 20.5°C in 6 days, whereas measured temperature rise was 17.6°C. For comparison, two‐dimensional heat transport through a fracture of uniform aperture (i.e., homogeneous permeability) predicted roughly 670 days until production well temperature would rise 17.6°C. This suggests that the use of a fracture of uniform aperture to predict heat transport may drastically overpredict the thermal performance of a geothermal system. In the context of commercial geothermal reservoirs, the results of this study suggest that combined inert/adsorbing tracer tests could predict production well thermal drawdown, leading to improved reservoir monitoring and management.

Subsurface environments host diverse microorganisms in fluid-filled fractures; however, little is known about how geological and hydrological processes shape the subterranean biosphere. Here, we sampled three flowing boreholes weekly for 10 mo in a 1478-m-deep fractured rock aquifer to study the role of fracture activity (defined as seismically or aseismically induced fracture aperture change) and advection on fluid-associated microbial community composition. We found that despite a largely stable deep-subsurface fluid microbiome, drastic community-level shifts occurred after events signifying physical changes in the permeable fracture network. The community-level shifts include the emergence of microbial families from undetected to over 50% relative abundance, as well as the replacement of the community in one borehole by the earlier community from a different borehole. Null-model analysis indicates that the observed spatial and temporal community turnover was primarily driven by stochastic processes (as opposed to deterministic processes). We, therefore, conclude that the observed community-level shifts resulted from the physical transport of distinct microbial communities from other fracture(s) that outpaced environmental selection. Given that geological activity is a major cause of fracture activity and that geological activity is ubiquitous across space and time on Earth, our findings suggest that advection induced by geological activity is a general mechanism shaping the microbial biogeography and diversity in deep-subsurface habitats across the globe.