The aim of the study was to determine the degree of soil pollution in different forest types around Krakow, using biochemical, chemical and magnetic methods. In assessing the degree of soil contamination, the impact of the forest species composition by comparing the surfaces of broadleaf and coniferous forest stands was taken into account. Enzymatic activity was related to other measures of pollution, i.e., heavy metal concentration, content of polycyclic aromatic hydrocarbons and level of magnetic susceptibility. The study was conducted in southern Poland. Krakow was the central point around which the test surfaces were designated. In soil samples pH, soil texture and organic carbon, nitrogen, base cation contents, dehydrogenase and urease activities were determined. Additionally, heavy metal concentration, polycyclic aromatic hydrocarbons (PAHs) content and magnetic susceptibility were recorded. The obtained results confirm the high diversity of forest soil pollution around Krakow agglomeration. Significant differences in both the content of heavy metals, polycyclic aromatic hydrocarbons and values of the magnetic susceptibility were observed in the soils of forest complexes located in different directions away from Krakow and at various distances from Krakow. The level of contamination is related to the historical and current deposition of industrial and urban dusts from Krakow and Silesia. The type of stand had a considerable influence on the content of polycyclic aromatic hydrocarbons. A higher content of aromatic hydrocarbons was observed in the soils of deciduous stands. A high level of magnetic susceptibility was observed in the soils of coniferous stands. The differences in enzymatic activity between the research plots result from different contents of PAHs and different amount of soil organic matter resulting from the species composition.

Aging is a complex physiological process that poses considerable conundrums to rapidly aging societies. For example, the risk of dying from cardiovascular diseases and/or cancer steadily declines for people after their 60s, and other causes of death predominate for seniors older than 80 years of age. Thus, physiological aging presents numerous unanswered questions, particularly with regard to changing metabolic patterns. Urine proteomics analysis is becoming a non-invasive and reproducible diagnostic method. We investigated the urine proteomes in healthy elderly people to determine which metabolic processes were weakened or strengthened in aging humans. Urine samples from 37 healthy volunteers aged 19–90 years (19 men, 18 women) were analyzed for protein expression by liquid chromatography–tandem mass spectrometry. This generated a list of 19 proteins that were differentially expressed in different age groups (young, intermediate, and old age). In particular, the oldest group showed protein changes reflective of altered extracellular matrix turnover and declining immune function, in which changes corresponded to reported changes in cardiovascular tissue remodeling and immune disorders in the elderly. Thus, urinary proteome changes in the elderly appear to reflect the physiological processes of aging and are particularly clearly represented in the circulatory and immune systems. Detailed identification of “protein trails” creates a more global picture of metabolic changes that occur in the elderly.Electronic supplementary materialThe online version of this article (doi:10.1007/s11357-013-9562-7) contains supplementary material, which is available to authorized users.

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