Raphia hookerifruits are used for fishing in Nigeria due to their ichthyotoxic properties. This study investigated the toxic effects ofR. hookerion the reproductive capacity ofClarias gariepinus. The results from both short-term (96-hour test) and long-term (3-month sublethal test) bioassays revealed a linear relationship betweenR. hookeriextract dose and negative effects on the catfish. The percentage survival of both sexes of the catfish decreased with increasing extract concentration at short-term exposure, with LC50values of 600 mg/L and 800 mg/L for male and female, respectively. At long-term exposure, the reproductive capacity of 10–12-month-old male and female brood-stocks diminished at relatively higher concentrations ofR. hookerifruit extract, with the gravid females producing fewer and mostly unviable eggs. The fruit extract also affected the eggs’ hatchability and fry survival when the exposed gravid females were treated with pituitary hormone and sperms from unexposed males, while the exposed males were unable to sexually stimulate female brooders. Sperms and pituitary hormone from exposed males were infertile, leading to low percentage of hatched eggs and mortality of the few hatched fries within 24 hours. These results confirmed the ethnobotanical use of this fruit extract for fishing in Nigeria.