Leishmaniasis is a disease caused by parasites of the genus Leishmania and currently is expanding in all states. Treatment is problematic, given that the recommended drugs are difficult to administer and serious side effects. The present study aims to test synthetic compounds, developed by the group of Chemistry, USP / São Carlos. The evaluation of compounds that act on enzymes of metabolism was performed in promastigotes of Leishmania amazonensis in cultured murine peritoneal macrophages.The results showed that the 17 compounds tested, three IDs (71S, 130S and 195 are dehydrogenase inhibitors enzima diidroorotato -DHODH) were toxic to promastigotes of L. amazonensis-GFP, in assays counting under a light microscope and measuring fluorescence spectra-fluorometer, though only the ID of the ID 71S 130S were considered significant by statistical analysis. These inhibitors are reduced infection with amastigotes in cultured murine macrophages peritoneias but these were toxic to macrophage cultures. The compound ID 195 was toxic to macrophages at the higher concentrations. In another experiment it was shown that at concentrations below 195 the ID was not toxic to macrophages, but did not reduce the number of amastigotes inside macrophages.

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