The study was designed to investigate the effect of duration of frozen storage on the chemical, microbiological and sensory profile of Tilapia fish (Sarotherodun galiaenus) collected from a research pond of the Agricultural Development Project, Akure, Nigeria, subjected to sixty days of frozen storage and analyzed at intervals of ten days. Protein content (%) ranges from 43.70 +/- 1.17-60.65 +/- 2.40. Protein decreases with increasing duration of frozen storage with the fresh samples (not frozen) having the highest protein content (60.65 +/- 2.40) while the least (43.70 +/- 1.17) was recorded for fish samples that were frozen for sixty days. Similar results were obtained for the fat content (%) where the highest fat content (9.72 +/- 0.25) was recorded for the fresh samples and the least value was recorded for those stored for sixty days. Ash content (%) and moisture content (%) do not show any significant change during storage. Mineral composition (Fe, Ca, Mg, P and Zn in mg/100g) and iodine content (microg/100g) of the samples showed a slight change with respect to duration of storage. pH values ranges between 5.20-6.90 while the total coliform count range was between 3.0 x 10(3)-7.5 x 10(6) with increasing values, as the duration of storage increases. Sensory evaluation of the fish samples on storage revealed that quality of the fish samples with respect to taste decreases with increasing duration of storage with the best quality (texture, odor and color) when freshly prepared; and that better quality during storage is obtainable during the first ten days of storage. These, by implication simply mean that fish should be stored for a short period of time to retain the taste, and provide both the protein and fat at optimal level.

Aluminum cookware are widely used in many parts of the world. Data is increasing on the leaching of toxic metals from aluminum cookware into food and drink. In the present study, cytogenotoxicity of water boiled in three different aluminum pots (new, 3-year-old, and 6-year-old) in onion root tip's dividing cells was evaluated using the Allium cepa assay. The concentrations of Pb, As, Cd, Cu, Ni, and Al in the samples were also analyzed. Onion bulbs were grown in the boiled water samples, while tap water served as the control. Cytological and genetic analyses were carried out after 48 h, while analysis of inhibition of root length was carried out after 72 h. The results showed a significant (p < 0.05) cell proliferation and root growth inhibition compared with the control, which is dependent on the duration of use of the aluminum pots. The boiled water samples also caused modification of the root morphology as well as chromosomal aberrations which include sticky chromosomes, anaphase bridge, and disturbed spindle. The highest cytogenotoxicity was observed in the 6-year-old aluminum pot and the least in the new aluminum pot. Pb, As, Cd, Cu, Ni, and Al analyzed in the samples, with the highest concentrations in the 6-year-old aluminum pot, were believed to be responsible for the cytogenotoxicity observed in the A. cepa assay. The data of this study are indications that the aluminum pot-boiled water contains substances with the potential to be cytotoxic and cause mutations in somatic cells of A. cepa.

Background.With a population of over 165,000,000, growing at an average rate of 2.7% per annum and an economic growth rate of about 5.7% in the past five years, the market for refined petroleum products in Nigeria is growing. As a result, the number of filling stations is increasing.Objectives.The present study evaluated the reproductive and genetic toxicity of simulated leachate of soil from petrol, diesel and kerosene dispensing sites in a filling station using the murine sperm abnormality test, sperm count and bone marrow micronucleus assay.Methods.Simulated leachate of soil collected from petrol, diesel and kerosene dispensing sites in a filling station was intraperitoneally administered to mice at different concentrations. Bone marrow micronucleus assay was carried out after 5-days exposure, while sperm morphology assay was carried out 35 days from the first day of exposure. Alterations to hematological parameters were evaluated and physico-chemical analysis of the leachate samples was also carried out.Results.The results showed a significant (p<0.05) concentration-dependent increase in abnormal sperm cells and decrease in mean sperm count in all the samples tested. Increased induction of micronucleated polychromatic erythrocytes was observed in the exposed mice. Hematological analysis showed a significant (p<0.05) increase in the values of white blood cell count (WBC), lymphocytes, neutrophils, monocytes, eosinophils and mean corpuscular volume (MCV), while a significant (p<0.05) reduction in basophils, hemoglobin, mean corpuscular hemoglobin (MCH), packed cell volume and mean corpuscular hemoglobin concentration (MCHC) values were observed.Discussion.In the present study, simulated leachates from soil obtained from petrol, diesel and kerosene dispensing sites were shown to cause genomic disruptions in germ and somatic cells, and hematotoxicity in an animal model. These observed reproductive, genetic and hemato-toxicities are believed to be caused by the presence of lead, copper, mercury, polycyclic aromatic hydrocarbons, and benzene in the samples.Conclusions.This study showed the negative impact of petroleum products in the contamination of soil, with a capability of inducing genetic damage in somatic and germ cells of exposed plants and animals.Ethics Approval.The study was approved by the ethical committee of the Federal University of Technology, Akure, Ondo State, Nigeria.

Background. Bisphenol A (BPA) is known to alter sperm morphology, but information is limited on the most susceptible stage(s) of spermatogenesis, especially in mice. Objectives. This study investigated the reproductive, biochemical, and hematological changes caused by exposure to BPA in male albino mice. The genotoxicity of BPA to the six stages of spermatogenesis in mice was determined. Methods. Mice were exposed orally to BPA at 0.5, 1.0, 2.0, and 5.0 mg/kg bw doses for 5 days and assessed for sperm morphology after 35 days. Based on the result, the second group of mice was exposed to BPA at 1.0 mg/kg bw dose for 5 days, their spermatozoa were assessed for sperm morphology based on BPA exposure at the 6 maturation stages of spermatogenesis: spermatozoa, elongating spermatids, round spermatids, secondary spermatocytes, primary spermatocytes, and spermatogonia. Biochemical and hematological analyses of the blood of exposed mice were also carried out. Results. The results showed that BPA induced concentration-dependent, significantly (p&lt;0.05) increased sperm cell abnormalities at three of the four concentrations tested, with the exception of 0.5 mg/kg bw, in comparison with the negative control. The highest frequency of sperm aberrations was induced in spermatozoa exposed to BPA while at the primary spermatocytes. The order of induced sperm abnormality at the different stages of exposure was: primary spermatocytes &gt; elongating spermatids &gt; spermatozoa &gt; spermatogonia &gt; round spermatids &gt; secondary spermatocytes. The results of the biochemical analysis showed significantly (p&lt;0.05) increased serum urea, creatinine, and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities with a concomitant decrease in total protein content at the various stages of spermatogenesis. In addition, the results for hematological parameters showed several significant (p&lt;0.05) modulations in mice exposed to BPA. Conclusions. These data showed that BPA is most toxic to primary spermatocytes and alterations of biochemical and hematological parameters might be the mechanisms of induced toxicity. Ethics Approval. The Research Ethics Committee, Federal University of Technology, Akure approved the study protocols. Competing Interests. The authors declare no competing financial interests

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