Food preservation is a big challenge for the industries. The mechanisms of preservation involved microorganisms such as lactic acid bacteria. Modeling the microorganisms growth is a very useful information to well-understand their behavior and to propose the best conditions for food preservation. In this way, this study was to propose a mathematical model of the dynamical growth of Lactococcus lactis CWBI-B1410 which play a main role for the bio-conservation of fish. This study is conducted by varying the concentration of glucose and nitrogenized matter. The results showed that the mathematical model predicted the growth of L. lactis CWBI-B1410. The curves that are predicted are exactly those that can be observed experimentally. The results showed that the production of biomass by L. lactis CWBI-B1410 is obtained on the MRS medium composed of 15 g of glucose per liter and 40 g of nitrogenous material per liter. From the obtained results, it could be clearly advocated that for two tests with the same amount of nitrogenous matter, it is possible to achieve economies of scale in glucose while maintaining the same biomass performance.