Background and Aim: Disposable imported nylon bags used in an in situ digestibility measurement restrict the effort of scientists to obtain more accurate information about ruminant feed quality due to their low affordability and environmentally unfriendly characteristics. This study aimed to find reusable local nylon fabrics to substitute imports. Materials and Methods: Five local fabrics (B1 = Abutai, B2 = Taffeta, B3 = Organza, B4 = N57, and B5 = M100) were used to make nylon bags and compared with the imported bag (B6 = Ankom technology). The research consisted of three steps: (1) Observing the similarity of the local nylon bag's hole number to the imported bag. (2) Testing feed in situ degradation (F1 = Napier grass, F2 = Cornmeal, and F3 = Dairy cattle total mixed ration) using bags B1-B6. (3) The reusability of the bag was tested using different washing methods (under running water [R1], rinse [R2], and ultrasonic water bath [R3]).Results: It was shown that the hole numbers of B1 (1223 hole numbers) and B2 (1245 hole numbers) were not significantly different from B6 (1248 hole numbers). It was shown with dry matter degradability measurement using the in situ method that there was no significant difference in feed solubility (a), potential degradability (b), and the effective degradation between local fabrics (B1-B5) and B6. According to the degradation rate (c), there were interactions between the feeds and fabrics. For F1, all local fabrics were similar to B6, while for F2, only B1 was different from B6. For F3, only B5 was different from B6. It was also shown by the organic matter degradability measurements that there was a similar trend. The exception was the solubility (a) component in F3, in which it was shown that B1 was also different from B6. It was shown in the reusable test that there was no difference in the weight of the bag before and after all washing methods. In contrast, the hole number increased due to the shrinking of the bag after drying in a 60°C oven. Conclusion:According to this in situ study, local nylon bag B2 can substitute imported bags. A lower drying temperature is suggested to prevent shrinking and make the bag reusable.