Background: Hepatitis B(HBV) is one of the most important occupational hazards among the medical student and healthcare workers(HCW's) due to risk of exposure to infected bodily fluids and blood. This study aimed to assess status of HBV vaccination and factors affecting anti-HBs titres levels for HBV in HCW and medical students. Material and Method: It was a prospective, descriptive and cross sectional study done at the Department of Microbiology, DRPGMC Kangra at Tanda(H.P). Randomly 215 HCW’s and medical students were taken in the study who fulfilled the inclusion criteria. Results: Total of 215 subjects were included in the study. Males were 89 and females were 126. 182 subjects were vaccinated and 33 were unvaccinated for hepatitis B. Out of 182 vaccinated 137 were completely vaccinated. Significant correlation was seen with decrease in antibody titers with gender, duration of vaccination and smoking. Conclusion: Vaccination should be mandatory for every HCW and medical student entering the health institutions. The factors affecting the antibody titers must be evaluated and antibody titers must be reviewed from time to time. The need for a booster dose can be made mandatory at least for healthcare professionals. Keywords: Hepatitis B virus, Vaccination, Antibody titers, HCW.

In most parts of India, water table is getting lowered. The problem is more predominant in dense cities. Some cities have become dependent on potable water supply. Due to paving of roads and construction of buildings, Infiltration has reduced and run off has increased. Run off increases when the rainfall is intense and with increased road and building infrastructure. Further there is huge withdrawal of water to meet the demand for growing population. Sowing of paddy to increase the returns from farming adds to the problem. Paddy needs 150cm of water from sowing to harvesting. It is not the natural crop of this region. Part of this water demand is met by monsoon and the rest by pumping out of water. There is huge and rapid withdrawal of water from tube wells to keep the crops inundated in water. This causes the drawdown or lowering of water table.  In the last two decades water has been receding at the rate of 50-100cm per year. After concerns of water harvesting were raised, the rate of decline has receded. Since we cannot do much about reducing water demand, the solution lies in water harvesting and recycling waste water. Roof top water harvesting is easy and has no challenges. Storm water harvesting is challenging and daunting task. The harvesters get clogged and may not be operational for long time. Optimum solution has been found for filter material to be used, and design of harvester.

Background: Hepatitis B(HBV) is a blood borne virus and it is one of the most important occupational hazards among healthcare workers (HCWs) & Medical students. This study aimed to measure the anti-HBs titres and to assess the seroprevalence of Hepatitis B in HCW and medical students. It was a prospective,descriptive and cross sectional hospital base Material and method: d study. Medical students and healthcare workers who had received all three doses of hepatitis B vaccination were included in the study. A total of 200 subjects , aged between 18 and Result: 62 years were taken. 182 were vaccinated and 18 were unvaccinated. 85 were males and 115 females. Seroprevalence of Hepatitis B was nil. Antibody titres were >100mIU/ml in 51.6% , 10-100mIU/ml in 16.4% & <10 mIU/ml in 31.8% respectively.Negative correlation of decreasing antibiotic titer and age was seen. HCW and medical students are at hi Conclusion: gher risk due to their continuous exposure to blood products. Vaccination should be compulsory to the HCW and medical workers in the institution as Hepatitis B is a preventable disease. Monitoring of antibody titers should be done from time to time to see waning off antibody titers after vaccination

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Introduction: Emerging resistance in microorganisms is a matter of great concern in health care setups. Bacterial isolate and AMR pattern changes from place to place and time to time. Unprofessional use of antibiotics has resulted in an increasing burden of antibiotic resistance. This study focused over the aerobic bacteriological profile and antibiotic resistance pattern in pus isolates. Materials and Methods: A retrospective study was carried out from June, 2020 to May 2021. 1174 pus samples collected during the study period were included. The samples were cultured on Blood and MacConkey agar. After aerobic incubation at 37°C for 18-24 hrs, organisms were identified by biochemical reactions, gram staining and antibiotic susceptibility was tested by Kirby Bauer disc diffusion method. Result: Among the total 1174 pus samples, 712 (60.6%) were male and 462 (39.3%) were females. 857(72.9%) samples showed positive results. Among them, Staphylococcus aureus was the most common isolate followed by Escherichia coli, Pseudomonas spp. and Klebsiella spp. The sensitivity of Staphylococcus aureus was highest with Vancomycin and Linezolid, whereas Enterobacterales and Pseudomonas were sensitive with Piperacillin tazobactam. Conclusion: Increasing resistance always poses a challenge in treating infections. The development of resistant strains of pathogens can be limited by the judicious use of antibiotics. This study shows the infecting bacterial isolates and their antibiogram from this area. Keywords: Pus, antimicrobial resistance, bacterial isolates.