Coccidiosis is an intestinal disease caused by a parasite of the genus Eimeria. This parasite mainly affects poultry species and causes great economic losses in the poultry industry. This study was designed to estimate the prevalence of coccidiosis in the local breed of domestic chicken in Nineveh Governorate, Iraq. 450 faecal swabs and intestinal samples (intestinal scraping) were collected from different local breeds of home-bred chickens from October 2020 to the end of March 2021. All fecal samples were examined using the flotation method by using sugar solution, and Eimeria was confirmed by the polymerase chain reaction method. Fecal examination results showed that 32.6% of the total samples were positive for Eimeria oocysts, classified into six species including E. brunetti, E. mitis.E. maxima E. acervulina E. necatrix, E. tenella with infection rates are 57.5, 44.2, 42.1, 26.5, 20.4, 16.3%, respectively. The phenotypic results were genetically confirmed by the result of the reaction of 455 base pairs. The prevalence of coccidiosis was highest in chickens less than three months of age 49.2% and lowest in chickens older than 6 months 23.9%.

In last ten years, there has been a developing enthusiasm for microscopic organisms from the genus Anaplasma, particularly the species A. ovis. It is associated with the pathogenic action of these microscopic organisms in livestock. Anaplasma ovis is a tick-borne obligate intracellular rickettsial bacterium that causes anaplasmosis in domestic and wild small ruminants. The samples of the present study were collected from small ruminants from inside seven distinct regions (Akre, Simele, Zummar, Feshchapoor, Deraboon, Bajed Kandal,Karoda)of Duhok province, 389 (goats 75 and sheep 314) during the period of April and May 2018, blood sample were taken and thin smear was formed, after Giemsa’s staining the slide is observed under microscope. In this study used Giemsa stain for microscopic examination out of 389 animals 250 were found positive for Anaplasma ovis infection with a prevalence rate of 64.26 % and 139 of them were negative with a prevalence rate of 35.73 %. According to the species of animals, the highest prevalence of A. ovis infection in animals by using microscopic examination was 67.83 %, 213 positive sample from total 314 blood samples from sheep and lowest prevalence was 49.33 %, 37 positive sample from total 75 blood samples from goats. PCR analysis of 100 blood samples obtained from total 250 positive blood samples after DNA extraction and measure of concentration and purity we used 2 primers that target major surface protein 4 (MSP4) in A. ovis genomic DNA. The results of PCR test with major surface protein 4 primer was 83 samples positive from total 100 samples, According to the species of animals, the highest prevalence of A. ovis was 83.7 %, 72 positive sample from total 86 blood samples from sheep and lowest prevalence was 78.5 %, 11 positive sample from total 14 blood samples from goats.