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Frequency of Rh and Kell system in blood donors of blood center in SantaMaria -RS ABSTRACT:The study was aimed at evaluating the frequency of Rh and Kell system phenotypes in donors at the Blood Center of Santa Maria. Frequency of such systems depends on each regions ethnicity. The main antigens of the Rh system include: D, C/c/Cw and E/e. From the 1832 phenotyped samples, regarding the Rh system, 870 (48%) were Rh positive and 962 (52%) were Rh negative. Frequency of the antigens was: cc 59.20%; Cc 27.67%; CC 12.55%; Cw 0.87%; ee 83.62%; Ee 15.30%; EE 1.08%; and 7.36% kell positive. From the samples phenotyped as Rh negative, 78 (8.1%) were positive for the "C" and/or "E" antigen and 8.2% were kell positive. Since these antigens may cause Severe hemolytic disease and Alloimmunization, it is required to search for them in the Rh negative donors.