Vascular endothelial growth factor A (VEGFA) plays a key role in the angiogenesis of human skin. Elevated levels of VEGFA are associated with several pathological conditions, including chronic inflammatory skin diseases and several types of skin cancer. In particular, squamous cell carcinoma (SCC) of the skin, the second most common skin cancer in the general population, is characterized by invasive growth, pronounced angiogenesis and elevated levels of VEGFA. The processing, turnover and production of VEGFA are extensively regulated at the post-transcriptional level, both by RNA-binding proteins and microRNAs (miRNAs). In the present study, we identified a new miRNA recognition element in a downstream conserved region of the VEGFA 3′-UTR. We confirmed the repressive effect of miR-361-5p on this element in vitro, identifying the first target for this miRNA. Importantly, we found that miR-361-5p levels are inversely correlated with VEGFA expression in SCC and in healthy skin, indicating that miR-361-5p could play a role in cancers.

Background:The identification of G protein-coupled receptors (GPCRs) specific for the lymphatic endothelial cells (LECs) is essential for establishing drugs targeting the lymphatic system. Results: GPR97 is an orphan adhesion GPCR that regulates LEC migration. Conclusion: GPR97 is the first known adhesion GPCR involved in lymphatic remodeling. Significance: This first evidence that adhesion GPCRs govern LEC motility opens new possibilities for modulating lymphangiogenesis.