Shallots have been used for generations as traditional medicine, one of which is used in the treatment of diarrhea. This study determinedthe antidiarrheal activity of the shallot bulbs using intestinal protection and intestinal transit methods, and to determine the effective concentration that gives the best antidiarrheal effect in mice. This study used thirtymiceanddivided into six groups.The negativecontrol groupwas givenTween 80 (1%), positivecontrol group was given LoperamideHCl, and four various concentrations of shallot infusion treatment groups (1.25%, 2.5%, 5%, and 10 %). The treatment was given by the oral route. The parameters used in the intestinal protection method were frequency of defecation and fecesconsistency, while in intestinal transit method the parameters used were the percentage ratio of the marker trajectories compared to the overall length of the intestine. The results showed that the 1.25% concentration of shallot infusion indicatedthe best antidiarrheal activity and had a smaller ratio or stronger inhibition tointestinal peristalticcompared toall other concentrations, 2.5%, 5%, dan 10%. We concludedthat shallot bulbs infusion with a concentration of 1.25% has the most effective anti-diarrheal effect. This research can be continued by measurement of other diarrheal parameters such asstool weight, as well as start and stop time of diarrhea to further clarify the anti-diarrheal potential of shallot bulb infusion