Background: Obtaining healthy sleep is important for both physical and mental health, improving productivity and overall quality of life, especially, among girls with primary Dysmenorrhea. Aim of the study: This study aimed to explore the effect of sleep hygiene practices on quality of sleep pattern, insomnia and academic achievement among nursing students with primary Dysmenorrhea Design: Quasi-experimental research design. Sample: A purposive sample of 106 female nursing students. Setting: This study was carried out at Faculty of Nursing, Mansoura University, Dakahlia Governorate, Egypt, from March 2021 to May 2021. Tools: Data was collected through six tools, basic data structured interview schedule, Pittsburg Sleep Quality Index (PSQI), Insomnia Severity Index, Scale of Positive and Negative Experience (SPANE), Flourishing Scale and Sleep hygiene practices questionnaire. Results: This study reported highly statistically significant differences between the study and control groups after intervention in terms of sleep quality, severity of insomnia, subjective feelings of well-being and ill-being, psychological well-being, academic achievement and sleep hygiene practices (P=<0.0001). Conclusion: this study concluded that receiving sessions about sleep hygiene practices has a greater significant impact on experiencing good sleep quality, less insomnia, more positive feelings of well-being and less negative feelings of ill-being, more psychological wellbeing, better academic achievement, and better sleep hygiene practices among nursing students with primary dysmenorrhea.