Platelet sensitivity to ADP and adrenaline was determined after storage of platelet-rich plasma (PRP) under various conditions to establish those yielding optimal platelet stability. The effects of the exclusion of air from the storage syringes, temperature, PRP dilution and duration of storage were tested. Storage at room temperature (22° C) in the absence of air stabilised PRP pH over 24 h and stabilised platelet sensitivity to ADP up to 4 h. Storage at 4°C and 13 C caused platelet activation and eventually spontaneous aggregation, as evidenced by significant reductions in platelet counts. Samples stored at 37° C were less responsive to ADP and adrenaline than samples maintained at 22 C. Platelet count adjustment to 200 × 10(9)/L reduced platelet sensitivity as reflected by increased agonist EC(50) values and threshold concentrations. Positive correlations between agonist EC(50) values (and between threshold concentrations) for diluted and undiluted samples were obtained, indicating that platelet count adjustment did not affect the ranking order of platelet sensitivity within the subject group. No correlations between platelet count and indices of platelet sensitivity were seen suggesting that differences in platelet aggregation arise from intrinsic differences in platelet sensitivity rather than differences in platelet count. With time of storage the responses to ADP (EC(50) and threshold concentration) and adrenaline (EC(50)) declined to a greater extent for undiluted PRP than for diluted PRP. No changes in the platelet-poor plasma concentrations of the dense granular component, serotonin, occurred in diluted or undiluted samples over 24 h. We conclude that in order to ensure optimal stability of platelets, PRP should be stored at room temperature (22°C) in the absence of air and tested within 4 h of preparation. A decision on platelet count adjustment is also required dependent upon the experimental objectives.

Objective: To study on clinical profile & maternal - fetal outcome of eclamptic patient.Methods: A prospective cross sectional study was done in the department of Obstetrics & Gynaecology in Chittagong Medical College and Hospital from January to December 2010. All patients with eclampsia were included in the study, it was 416. Patients came with convulsion  other than eclampsia e.g. epilepsy, malaria, septicemia, meningitis, encephalitis, cerebral haemorrage, high fever, hepatic coma were excluded.Main outcome measures: Incidence of eclampsia, sociodemographic status, ante natal  care, time interval between attack and admission, level of consciousness was assessed by AVPU(Alert, response to voice, response to pain stimuli, Unconsciousness) score, types of eclampsia patients (antepartum,intrapartum,postpartum), number of convulsion, gestational age distribution of the patients, mode of delivery , maternal and fetal outcome.Results: Total number of deliveries during this period was 13,635. The incidence of eclampsia in this study was 3.05 %. Among 416 patients with eclampsia most of the patients were between 20-25 years (77%), a large number were primi para (72.5%), most of them comes from rural area (76%), most of them belongs to poor socioeconomic condition (72%), 49% patients were illiterate, 60 % patients had no antenatal check up, 52 % patients came after 6 hours of beginning of convulsion, 18 patients (4%) were unconscious, most of the patients had antepartum eclampsia (64%) , number of convulsion was between 5-9 in about 58% case , 63% were delivered by LSCS, 23% mother showed complications of eclampsia, of them pulmonary oedema (7.45%)  and renal failure(6.49%) were common, 35 (8%) mothers were died. Among perinatal mortality 18% baby were stillbirth and 9% were early neonatal death.Conclusion: Eclampsia is still a major killer disease in Bangladesh. It is a preventable  disease if preeclampsia is diagnosed by antenatal care. By giving quality antenatal care, mass awareness regarding the importance of antenatal care, emergency obstetric service in  the upazilla health complex we can prevent eclampsia. Female education, employment,  empowerment is urgently needed to reduce the incidence of this killer diseases. DOI: http://dx.doi.org/10.3329/bjog.v26i2.13784 Bangladesh J Obstet Gynaecol, 2011; Vol. 26(2) : 77-80

Background: All women go through menopause stage after a certain age and menopause women suffer from different medical problems which needs specific attention. Aim: The aim of the study is to find out the consequences of postmenopausal women attending in the hospital for medical treatment. This study also identifies the outcome of different consequences in those patients. Methods: Among 105 patients were selected for the study purposively those who were admitted in the gynecological ward in Kumodini Women's medical college from March 2008 to February 2009. All patients were undertake clinical examination and find out the consequence of menopause in one year observation period. A structured questionnaire was used to interview the patient and information also collected from patient's treatment file. Statistical analysis was performed by using statistical software SPSS 11.5 window. Results: Among 105 patients, age was above 40 years. Most of the patients were coming from low and low middle income society having maltiparous We have examined that most of the menopausal women developed uterovaginal prolapse was 63.8%, genital malignancies was found among 17.4% patients and rest were faced other benign disorders (19%) which includes leiomyoma, pelvic inflammatory diseases (PID), uncontrolled bleeding, urinary tract infections (UTI) and ovarian cysts. Conclusions: In Post menopausal phase of women in Bangladesh suffered from different health consequences like uterine prolapse, benign diseases and carcinoma which causes of hospital admission for better management. KYAMC Journal Vol. 3, No.-2, January 2013, Page 290-293 DOI: http://dx.doi.org/10.3329/kyamcj.v3i2.15169

Cervical ectopic pregnancy is the implantation of the conceptus within the cervix below the level of internal os 1 . Such pregnancy typically aborts within the first trimester, if it is implanted closer to the uterine cavity called cervico isthmic pregnancy it may continue longer 2 . Cervical pregnancy accounts for less than 1% of all ectopic pregnancies, with an estimated incidence of 1 in 2500 to 1 in 18000. Though the pregnancy in this area is uncommon but possibly life threatening condition due to risk of severe hemorrhage and may need hysterectomy 2,3 . Early detection and conservative approach of treatment limit the morbidity and preserve fertility. A 37 year old lady para 4+0 diagnosed as a case of cervical ectopic pregnancy with intractable bleeding and save the patient by emergency hysterectomy to control hemorrhage. The case is reported here for its relative rarity.

Objectives of this study are: 1. To find out the number of facitilities providing EmOC services in rural areas of Chittagong district. 2. To assess the proportion of women who deliver at Emoc facilities. 3. To find out the “METNEED” at EmOC facilities. 4. To find out the caesarean deliveries as a proportion of all births at EmOC. 5. To see the “Case fatality rate” which reflects the quality of care & facility performance. This is a retrospective study between January 2009 to December 2009 done in thirteen upazilla health complexes in Chittagong district of population size-52,39,000. Outcome measures are availability of EmOC, Proportion of births in EmOC facilities, Met need, Cesarean deliveries &case fatality rate. About 6.7 & of births take place in Comprensive EmOC facilities and 2.4% in Basic EmOC (i.e. About 9.1% births are institutional). Study shows that “Met Need” is about 18%. Only <0.8% of all births in the population is delivered by casesarean section. In this study case fatality rate is only .067%. This study describes the baseline indicates calculated in different upazillas. In Chittagong only 5 Comprehensive EmOC services are not sufficient to cover the largely populated area. If we expand the Basic EmOC and Comprehensive EmOC we can help the people even in grass root level. JCMCTA 2012; 23(1): 7-10