Gold–polymer nanostructured composites have a great potential in the biomedical and advanced materials field as an antimicrobial agent against various pathogens, especially viruses. In the present work, gold and gold-PVP colloids have been prepared by the electrochemical reduction of hydrogen tetrachlorauric acid (HAuCl4·3H2O) precursor. The atmospheric pressure microplasma technique was used as a reducing agent, while D-Fructose was used as a stabilizing agent in the synthesis process. X-ray Diffraction (XRD) confirmed the crystalline behavior of both gold nanostructured particles and gold-PVP nanocomposites. The morphology of the nanoparticles was examined by SEM. The absorption characteristic peaks at 541 nm and 542 nm in UV-Vis spectra confirmed the surface plasmon resonance in gold nanostructured particles and gold-PVP nanostructured composites, respectively. Dynamic light scattering studies with percentage intensity distribution revealed particle size distribution ranges from 8–288 nm for gold colloids and 15–297 nm for gold-PVP colloids. Gold-PVP nanostructured composites have shown an improvement in the antiviral activity against the H9N2 virus as compared to bare gold nanostructured particles.

In the present project, fructose-stabilized gold, silver and gold–silver bimetallic colloids have been synthesized by the electrochemical reduction of HAuCl4·3H2O (Au precursor) and AgNO3 (Ag precursor), employing the atmospheric pressure microplasma technique. X-Ray Diffraction patterns of gold–silver bimetallic particles exhibit (111), (200) and (220) planes identical to gold and silver NPs depicting FCC structures. The decrease in the peak intensities of Au–Ag (111) and Au–Ag (200) as compared to those of Au (111) and (200) is due to the formation of Au–Ag alloys. The FE-SEM image of gold–silver bimetallic NPs has revealed an adequate change in morphology as compared to the morphology of gold NPs and silver NPs. The majority of the gold–silver bimetallic NPs are spherical and are uniformly dispersed. The EDS spectra of (Au–Ag) confirm the presence of metallic gold and silver. The appearance of a single Surface Plasmon Resonance (SPR) peak in the UV–VIS absorption spectra of gold–silver colloids and its position in between the SPR peaks of the UV–VIS absorption spectra of gold and silver colloids justify the formation of gold–silver bimetallic alloy particles. In DLS measurements, the size distribution of gold–silver bimetallic colloids carries a narrow range 55 to 117 nm as compared to the size distribution of gold and silver colloids. The compatibility of the sizes of these colloids and the influenza virus belonging to the Orthomyxoviruses family (size range 80–300 nm with different morphologies) are assumed to stand responsible for an effective bio-conjunction with Influenza viruses. Au–Ag bimetallic nanostructures have synergistically improved their antiviral activity against H9N2 influenza virus as compared to monometallic AuNPs and AgNPs. Thus, the Au–Ag nanostructured alliance has been proven to be more effective and is capable of manifesting high antiviral efficacy.