Pseudomonas aeruginosa, Acinetobacter spp. and Klebsiella spp. are three of the pathogens most frequently involved in infections of cancer patients, and the production of b-lactamases is a major mechanism of resistance due to its wide diversity of existing enzymes. Therefore, the aim of the present study was to investigate the microbiological profile and data related to patients and infections, and to search for b-lactamase genes in bacterial isolates from hospitalized cancer patients in a hospital in Recife, Pernambuco, Brazil. A total of 169 isolates were recovered between 2012 and 2014, of which 58 were P. aeruginosa, 36 were Acinetobacter spp. and 75 were Klebsiella spp. A high percentage of carbapenem resistance was observed in P. aeruginosa and Acinetobacter spp. Among the carbapenem-resistant bacteria, the bla SPM-1 gene was detected in P. aeruginosa (35.5 %) and Acinetobacter spp. (3.8 %), while bla KPC was detected in P. aeruginosa (25.8 %) only. Among the third-and fourth-generation cephalosporin-resistant strains, in Klebsiella spp. we detected the genes bla TEM (30.6 %), bla CTX-M (58.3 %) and bla KPC (5.6 %), and in Acinetobacter spp. only bla TEM (25.9 %). This the first report of an Acinetobacter baumannii bla SPM-1 gene carrier that has been isolated in Brazil. The most frequent cancer types were bowel tumour [14.8 %; 95 % confidence interval (CI 95 % ) 9.8-21.1 %], breast cancer (13.6 %; CI 95 % 8.8-19.7 %) and prostate cancer (11.2%; CI 95 % 6.9-17.0 %). These results therefore provide knowledge of susceptibility profile and resistance mechanisms and thus can contribute to the strategic formulation of hospital infection control plans and the rational use of antimicrobials, reducing mortality from infection levels in cancer patients. frequency and severity of infections may be related to the use of potent cytotoxic drugs and increasingly invasive diagnostic tools (Maschmeyer & Haas, 2008;Wang et al., 2011). INTRODUCTIONGiven this vulnerability among patients with cancer, this population is more susceptible to community and healthcare-associated infections (HAIs). According to a recent survey by the Centers for Disease Control and Prevention (CDC), one in 25 hospitalized patients [4.0 %; 95 % confidence interval (CI 95 % ), 3.7-4.4] is battling an HAI, defined as those infections acquired in hospital or healthcare service unit that first appear 48 h or more after hospital admission or within 30 days after discharge following in-patient care (Revelas, 2012;Magill et al., 2014). Other causes for concern in many healthcare centres for patients with cancer are the high bacterial resistance rates and the rise of opportunistic multidrug-resistant (MDR) pathogens such as Pseudomonas aeruginosa and Acinetobacter baumannii (Pongas et al., 2012;Fukuta et al., 2013;Marín et al., 2014).Gram-positive bacteria are described as the most frequent cause of HAIs in cancer patients, especially through bloodstream infection (Wisplinghoff et al., 2003;Maschmeyer & Haas, 2008;Wang et al., 2011; Kosmidis & Chandrasekar...

Esse estudo objetivou avaliar o leite in natura comercializado em Caruaru, localizada no Nordeste do Brasil, através de ensaios físico-químicos e bacteriológicos. Os testes físico-químicos foram baseados e interpretados de acordo com a Instrução Normativa 69/2006 e a comparação dos resultados foram realizadas de acordo com a Instrução Normativa 62/2011 do Ministério da Agricultura, Pecuária e Abastecimento. A investigação bacteriológica incluiu a pesquisa e estimativa do número mais provável de coliformes totais e fecais, Pseudomonas aeruginosa, e contagem de Staphylococcus aureus. A presença de sangue foi evidenciada em 6,7% das amostras e 70% apresentaram positividade para a presença de exsudato inflamatório. A presença de coliformes totais foi reportada em todas as amostras e 96,6% apresentaram positividade para coliformes fecais. Um total de 70% das amostras apresentou-se inapropriado para o processo de pasteurização e todas foram positivas para a presença de S. aureus, cuja contagem variou de 7 x 103 a 1,8 x 106 UFC/ml. As amostras analisadas foram também positivas para P. aeruginosa, das quais 90% destas apresentaram NMP/100ml>110. O leite in natura comercializado em Caruaru possui baixa qualidade sanitária e apresenta-se inapropriado para consumo humano. A facilidade de aquisição do produto demonstra a necessidade de maior vigilância pelas agências específicas.

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