Entamoeba gingivalis -prevalence and correlation with dental caries in children from rural and urban regions of Lublin Province, Eastern Poland. Ann Agric Environ Med. AbstractIntroduction. Entamoeba gingivalis, as the name implies. typically lives around the gumline of the teeth in the tartar and gingival pockets of the oral cavity. Pathogenicity of protozoa in the oral cavity is not completely understood. The occurrence in patients with a good state of the immune system usually does not cause any pathological changes. In the presentede survey, an increased incidence of Entamoeba gingivalis in conjunction with pathological changes in the oral cavity was found. Objectives. The aim of the study was to evaluate the prevalence of Entamoeba gingivalis in children treated in Department of Paediatric Dentistry of the Medical University in Lublin and its correlation with dental caries. Materials and method. Diagnostic materials in the form of swabs were obtained from 154 children in the age 2-18 years old, treated in the Department of Paediatric Dentistry of the Medical University in Lublin. The main index for epidemiological surveys in oral health -DMFT (decayed, missed, and filled teeth) for permanent teeth and DMFT for deciduous teeth was calculated.Results. The average number of Entamoeba gingivalis in urban children was 12.84. The DMFT number in the deciduous teeth was lower in urban patients (5.0) than from rural patients (5.4). The DMFT number in the permanent teeth was lower in urban patients (3.155) than rural patients (3.98). There was no statistically significant correlation between the DMFT value and the number of Entamoeba gingivalis. Conclusions. 1) Entamoeba gingivalis occurs in the oral cavity of children. 2) In the presented survey, both DMFT and dmft indexes were high which proves high activity of dental carious. 3) There was no significant statistical correlation observed between dental caries and the presence of Entamoeba gingivalis.

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Dental caries is listed by the WHO as one of the major non-communicable diseases that need to be prevented and treated. The aim of the study was to evaluate the prevalence and severity of caries expressed as the Decayed, Missing and Filled Permanent Teeth (DMFT) index in 12-year-old Polish children and to verify bacterial species related to the occurrence of dental caries. Quantitative real-time PCR analysis of DNA isolated from saliva samples was performed to detect 8 cariogenic and periopathogenic bacterial strains. A total of 118 Polish children were enrolled in the study. They had low mean DMFT scores of 1.58 ± 1.98. The prevalence of dental caries in the children tested was low (53.4%), with a tendency to decrease compared to previous oral surveys. Bacterial abundance of other species in the dental caries and caries-free groups did not differ; however, periopathogenic Prevotella pallens, Fusobacterium nucleatum along with cariogenic Streptococcus mutans and Lactobacillus fermentum were significantly strongly correlated in the caries-active subjects. The prevalence of S. sobrinus was significantly higher in children with dental caries (p = 0.023) and correlated with higher DMFT. It may temporarily play an important role in the initiation of the cariogenic process or in its enhancement due to an ecological imbalance in dental microbiota.

Dental anxiety is a very important factor affecting the efficacy of prevention, diagnosis and treatment of dental diseases, both in patients in the developmental age and in young adults. Anxiety is considered an emotional state with negative connotations. The aim of the study was to determine the level of dental anxiety in first year university students, the intent being to help to develop an individual treatment plan in this group of patients. The study involved 280 students including 230 females and 50 males. Of these, 122 patients were from the Medical University of Lublin, 79 from University of Life Sciences and 79 from University of Maria Curie-Skłodowska. The mean age of the study subjects was 21 years and 8 months ± 3,9 months. No differences in the level of dental anxiety between women and men were observed. The highest level of dental anxiety was observed among students of University of Life Sciences, while the lowest level was observed among students of the Medical University