BACKGROUND Chronic Obstructive Pulmonary Disease (COPD) is an important cause of disability and death globally and scenario has worsened. Acute Exacerbation of COPD (AECOPD) is associated with airway inflammation resulting in increased air trapping, further obstruction of airways and deterioration of gas diffusion. We wanted to determine the bacteriological pathogens causing acute exacerbation of COPD and their microbial sensitivity pattern. METHODS This is a cross sectional study comprising of 103 patients diagnosed with AECOPD. Before starting antibiotics, sputum samples of all patients were sent for culture and sensitivity. RESULTS Out of a total of 103 cases, 67% were males and 33% were females. Streptococcus pneumoniae was the commonest bacterium isolated (32.6%) followed by Klebsiella species (18.6%) and E. coli (16.3%). Highest sensitivity was found to be of Colistin and Polymyxin-B (100.0%) followed by carbapenems (86.0%) and Amikacin (74.4%) while the highest resistance patterns was of Azithromycin (32.6%) followed by Oxacillin (65.1%) and Tobramycin (62.8%). CONCLUSIONS AECOPD is more common in adult males above the age of fifty-five years secondary to smoking practices. There were no statistically significant correlation of age group, gender and smokers with positive sputum culture. Out of 103 patients, 41.7% had positive sputum culture and rest 58.3% had negative sputum culture report. Antimicrobials effective against gram-positive bacteria were Amikacin, Moxifloxacin, Carbapenems, Colistin and Polymyxin-B and therefore, the drugs of choice in treating AECOPD in our setting.