Background: Several processes in a teeth treatment often inflict wound in gingiva. Collagen is the key component of the wound healing. The collagen roles in advancing wound healing particularly, triggering the synthesis to induces collagen and cellular migration, and also inducing clotting cascades. Wound treatment using natural product is potential in accelerating wound healing, in particular duck egg membrane which contains collagen, glucosamine, and hyaluronic acid. This study aims to find out the influence of gel extract application of duck egg shell membrane 10 % towards collagen solidity on gingiva wound recovery process (study of Rattus norvegicus). Method: The subjects of the study consist of 20 male Rattus norvegicus aged 2-3 months which were given wound on labial gingiva of the maxillary central incisors with punch biopsy. The samples were divided into 2 groups, they were positive control group (Aloclair Gel