Emergence of multidrug resistance bacteria (MDR) in burn infections including Enterococcus yet to be managed and highlighted in most of hospitals in our region. This study included 200 clinical samples collected from wound, urine, stool and blood of 75 burn patients in Al-Faiha Teaching Hospital in Basrah, Iraq, between 2020 -2021. Based on Enterococci chromo agar, biochemical test and PCR for 16S rRNA gene, 50 isolates were identified as Enterococcus spp., involving 20 (40%) E. faecium, 14 (28%) E. faecalis, 7 (14%) E. gallinarum, 6 (12%) E. gilvus, 2 (4%) E. casseliflavus and 1 (2%) E. avium. All detected Enterococci were reported worldly as pathogenic bacteria to human. Six new local strains of Enterococcus were recorded in NCBI and the Gene bank as follow; Enterococcus gallinarum strain IraqYaHa5 and IraqYaHa19, Enterococcus faecium strain IraqYaHa23, IraqYaHa48 and IraqYaHa60, Enterococcus gilvus strain IraqYaHa50. Phylogenetic tree was constructed to all isolated Enterococci. The clinical isolates showed resistance up to nine antibiotics. However, enterococci isolated from healthy people during this study were 100% sensitive to six of those antibiotics. Duplex PCR was applied to detect vancomycin and gentamicin resistance genes. All resistant Enterococcus to gentamicin had harboured aph (3’).