The present study was designed to investigate the pathological lesions in different organs induced by chronic Ultraviolet type B (UVB) and Xylene exposure in mice. Forty mice were used in this study and divided into four groups; Group A (Control group, n=10) not exposed to UVB and Xylene, Group B (n=10) exposed to Xylene through painting the mouse's back skin, Group C (n=10) which were exposed to UVB light only, while the remainder group (Group D, n=10) were exposed to Xylene and UVB. The dorsal skin of mice from group B and D were painted with 1ml of Xylene whereas the animals in the group C and D exposed to UVB light for 30 minutes 4 days/week (6 weeks). The result showed that Xylene exposure in group B produced chronic tracheitis, bronchitis with some degenerative lesions in the neurons and glial cells of the brain, no specific lesions were found in the liver, spleen and kidney. UVB exposure in group C caused chronic tracheitis, lesions like lobar pneumonia, bronchopneumonia, and atelectasis, cellular swelling in the liver and kidney and follicular depletion in the spleen. In group D, the lesions became more prominent when animals exposed to both Xylene and UVB. We concluded that Xylene exposure has an adverse effect in some internal organs of mice, but not to all, when combined with UVB exposure it exaggerates its adverse effects on all of the examined organs.