No abstract

An androgen excess ovarian microenvironment may limit follicle progression in sheep. Two populations of ewes with divergent follicular fluid androstenedione (A4) were identified in a flock in Jordan: High A4; (A4) ≥ 30 ng/mL, (n=12) or Control A4 (Control); A4 ≤ 15 ng/mL; (n=12). We hypothesized High A4 ewes would have increased steroidogenic enzyme mRNA abundance, inflammation and follicular arrest. Messenger RNA abundance for steroidogenic enzymes StAR, CYP17A1, CYP11A1, and HSD3B1 were increased in theca cells while CYP17A1, CYP19A1 and HSD3B1 were increased in granulosa cells in High A4 ewes compared to Control. Gonadotropin receptor mRNA expression for LHCGR was increased in theca and FSHR in granulosa in High A4 ewes. Messenger RNA expression of FOS when reduced, increases expression of CYP17A1 which was observed in High A4 granulosa cells compared to Control. Furthermore, High A4 ewes had greater numbers of primordial follicles (P < 0.001) and fewer developing follicles compared to Control before, and after 7 days of culture, indicating follicular arrest was not alleviated by cortex culture. Increased fibrosis in the ovarian cortex was detected in High A4 ewes relative to Control (P < 0.001) suggesting increased inflammation and altered extracellular matrix deposition. Thus, this High A4 ewes population has similar characteristics to High A4 cows and women with polycystic ovary syndrome suggesting that naturally occurring androgen excess occurs in multiple species and may be a causative factor in follicular arrest and subsequent female sub- or infertility.

The objective of this study was to test whether prostaglandin (PG) injection on day 30 postpartum (pp) and detection of estrus can affect the efficacy of injecting PG on days 5 and 6 in the timed artificial insemination (TAI) protocol on pregnancy rate in a large dairy herd in hot or cold seasons. Out of 2235 cows, 1998 received an injection of PG at 30±3 d pp and estrus was observed. Cows that displayed estrus during the estrous observation period after PG injection were classified as estrus (E), while those that did not show estrus were classified as nonestrus (NE). Cows in each group were assigned to two treatments: CO-72 (control treatment) (ECO-72 and NECO-72) (day 44 GnRH, day 51 PGFnormal2italicα, day 54 GnRH+TAI) or PG–PG (EPG–PG and NEPG–PG) (day 44 GnRH, day 49 PGFnormal2italicα, day 50 PGFnormal2italicα, day 52 GnRH+TAI). Pregnancy was diagnosed on days 33 and 47 after artificial insemination (AI). The proportion of cows in estrus on the day of TAI was higher (P≤0.05) for cows that received two PG than for cows that received one PG. Pregnancies per AI (P/AI) on days 33 and 47 for cows inseminated during and after a voluntary waiting period in the NEPG–PG treatment had higher rates than for cows in the EPG–PG, ECO-72 and NECO-72 treatments. Moreover, P/AI were significantly (P≤0.05) affected by parity. Primiparous had higher P/AI (37.0 %) than multiparous cows (31.6 %). Cows inseminated in cold months had higher P/AI and reduced PL (35.6 % and 20.8 %) than cows inseminated in hot months (29.1 % and 30.6 %, respectively). In conclusion, treatments with PG on days 5 and 6 after the first GnRH injection increased P/AI. Estrus detection before the beginning of TAI protocol did not affect fertility. To maximize P/AI cows exhibiting heat at any time during the synchronization protocol should be inseminated.