This study was undertaken to assess the physical, chemical and hydrochemical quality of spring water in the southwestern basin of Jordan during a 60-month follow up monitoring study (April 2009 to April 2014. The samples were analyzed for temperature, conductivity, dissolved oxygen, pH, major cations, major anions and trace metals. The results show that there were considerable variations among the analyzed samples with respect to their physical and chemical parameters, which lie below the maximum permissible levels of the Jordanian and World Health Organization (WHO) drinking water standards. This study shows that the trace metals of spring water in the study area do not generally pose any health or environmental issues. The assessment of water samples indicates that groundwater, in general, is chemically suitable for drinking and agricultural uses.