Primary rainforest as an old heritage has become widely known because of its old natural, beautiful setting and the richest of its biodiversity. A nature-based trail is a natural path in the rainforest as a medium to explore the uniqueness of the natural beauty assets and the biodiversity of the forest. The development of the nature-based trail settings in a primary rainforest gives the impact towards visitor's experience indirectly to appreciate the biodiversity. Recreation Opportunity Spectrum (ROS) parameters will be used to interpret the effectiveness of the trail. The sustainability of natural resources becomes a major concern towards the management and conservation of the biodiversity.