Harapan forest is the remaining lowland tropical forest in Sumatra which represents about 20 percent of the biodiversity on the island of Sumatra. There are several Rasbora species found in the Sungai Kapas Tengah River Refuge in the Harapan Jambi Forest that the relationship is not yet known.This research aims to know kinship and genetic distance several species of Rasbora from Sungai Kapas Tengah, Hutan Harapan Jambi. The method in this research using secondary data from the NCBI website ((National Center for Biotechnology Information). The data taken, namelynucleotide sequence from the Cytochrome Oxidase I (COI) gene in mitochondrial DNA. The Rasbora species analyzed were Rasbora from identification results in the Sungai Kapas Tengah River Refuge, Harapan Jambi Forest, consisting of, Rasbora bankanensis, R. caudimaculata, R. cf. sumatrana, R. dusonensis, R. elegans, R. sumatrana, and R. trilineata. Based on phylogenetic analysis, the location of the branch length in each Rasbora species, the closest kinship is owned byR. sumatrana and R. elegans species. Based on the results of genetic distance analysis, the closest genetic distance was the species R. elegans and R. sumatrana, with a distance value of 0.023 (2.3%). While the farthest genetic distance between R. bankanensis and R. caudimaculata, with a distance value of 0.172 (17.2%).Based on research results It can be concluded that R. bankanensis has a greater kinship and genetic distance value than other Rasbora species, so that this species forms a separate group. Meanwhile, 5 other species have kinship and the value of close genetic distance so that these species are united in the same group. For future researchers, it is hoped that some additional families of fish species will be analyzed for phylogenetic analysis in Sungai Kapas, Hutan Harapan Jambi, so that they can find out the relationship of several other species.

This study aims to determine the quality of the final exam questions for the odd semester biology class XI MAN 2 Jambi City. The research subjects were 167 students of class XI MAN 2 Jambi City in the 2020/2021 academic year. This research is a descriptive study with data collection techniques through test instruments in the form of multiple choice questions, answer sheets, and answer keys. The data analysis technique used the Anates version 4.0.9 program to determine the reliability, level of difficulty, discriminatory power, distractor quality, and recap of item analysis. The results in this study indicate that the reliability value of the test items is 0.06 with very low criteria. For the item difficulty level, there are 4 questions of "very easy" difficulty, 2 questions "easy", 7 questions "medium", 4 difficult "difficult" questions, and 8 questions "very difficult". On the discriminatory power of items, there are 7 items classified into the "very bad" category, 8 items classified as "bad", 6 items classified as "enough", and 4 items classified as "good". While the results of the analysis of the quality of the item distractors, which have a distractor level of less than 51% which are generally found in each item. In addition, there are very few distractors in the percentage categories (51-75 or 126-150) and 76-125. Therefore, it can be concluded that, overall there are no questions that can be directly used, but some questions must be improved, and the rest cannot be used. There are 11 questions that need to be improved, and the remaining 14 questions that cannot be used.

Biology Olympiad is one of the national tests run annually. The quality of the item tests needs to be analyzed details. This study aims to determine the quality of the MAN (Public Islamic School) Model Biological Olympiad questions in Jambi City. The subjects in this study were 10 graders of MAN Model Jambi City who attended the Biology Olympiad training. The data analysis technique used in this study was a program called States 4.0.9. The results in the study showed that for the validity of the items at the coefficient level of 5%, there were 13 valid questions and 27 invalid questions. Meanwhile, for the reliability of the questions, the value was 0.46 which was categorized as sufficient. Furthermore, for the difficulty level of the questions, they were 3 questions in the easy category, 16 questions in the medium category, and 21 questions in the difficult category. Then for the results of the distinguishing power of the questions, 7 questions were categorized as very good, 12 questions were categorized as good, 7 questions were categorized as poor, and 14 questions were categorized as very poor. Meanwhile, for the results of the analysis of the quality of distracting items, there were 4 questions with quality was categorized as very good and good, while the rest were classified as poor, poor, and very poor criteria, or those criteria need to be revised.This is an open-access article under the CC-BY-SA license