Increasing the resistance to current antimicrobial agents has posed a pressing need for the development of new antimicrobial agents. We, therefore, benefit from the antimicrobial characteristics of pyrimidine derivatives to prepare a new 4-(4bromophenyl)-6-methyl-2-thioxo-1,2,3,4-tetrahydropyrimidine-5-carbohydrazide ligand. The ligand complexes of (3d; Cr, Mn, Fe, Co, Ni, Cu, and Zn) and 4d -Cd metals are also prepared and characterized by different techniques. The ligand acts as a neutral bidentate and exhibited octahedral geometry. In vitro, the antibacterial activity of the ligand and its complexes against Staphylococcus aureus and Escherichia coli was investigated. Also, the antifungal against Candida albicans and Aspergillus flavus was evaluated. The results showed that the produced complexes have antibacterial activity higher than the ligand. The Cd(II) complex showed antifungal activity higher than the other complexes.