The current study aims at investigating the morphology of pollen grain for eleven woody species of Fabaceae cultivated in New Damietta, Egypt. The pollen grains were prepared according to Erdtman's acetolysis method, described and photographed using light microscopy (LM). The results showed variations in their qualitative and quantitative features. Differences in pollen shape (subprolate, prolate, prolate-spheroidal, oblate-spheroidal), size (medium to large), pollen arrangement (monads or polyads), amb (circular, irregular, elliptic or triangular), polarity (isopolar, heteropolar), type of apertures (porate, colporate, inaperturate) and exine ornamentation patterns (psilate, verrucate, granulate, reticulate and psilate-perforate) are characterized.Electrophoretic (SDS-PAGE) examination of total pollen protein content of four species (Acacia ehrenbergiana, Cassia glauca, Delonix regia, Leucaena leucocephala) revealed distinct protein bands. The results of this study is of great importance in understanding the pollen types of some allergy producing species.
The pollen morphology of 36 woody species from the New Damietta City, Egypt. Belonging to 20 families of angiosperms, distributed in eighteen dicots and two monocots were investigated. The pollen grains were acetolysed, measured, described and illustrated under light microscopy (LM). Examination of these families revealed great pollen diversity in their qualitative and quantitative characters. The results revealed 58.3% of species with prolate-spheroidal pollen, 16.7% with oblatespheroidal pollen, 16.7% with subprolate pollen, 5.6% with prolate pollen and 2.7% with suboblate pollen. Tricolporate apertures accounted for almost half of apertures types; triporate, monocolpate, tricolpate, tetra-to penta-porate, tetraporate, pantoporate, inaperturate, tetracolporate and spiral aperture representing the remainder. Exine ornamentations ranged from psilate (33.3%), reticulate (27.7%) to micro-reticulate, scabrate, granulate, echinate each represents (2%) and clavate, verrucate, rugulate, striate, psilate-perforate, clavate-reticulate made up (1%) for each type. On the basis of pollen units, number and type of apertures, exine patterns were great diagnostic significance among the examined taxa. Six major pollen types and nine subtypes included in two groups (polyads and monads) were recognized. Description of pollen types, LM micrographs and a key for identification of the investigated taxa are provided.
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