Background: Neonatal sepsis is considered a major neonatal issue and associated with a high rate of mortality in newborns. Because there is a lack of studies concerning early hemodynamic changes of cerebral circulation in neonatal sepsis, so our research aims to assess the cerebral blood flow (CBF) changes in newborns with early onset neonatal sepsis (EONS) and to clarify the role of cranial Doppler Ultrasound in EONS diagnosis. Aim of The Work:To evaluate cerebral hemodynamic changes using transcranial Doppler in newborns with EONS. Patient and Methods: This cross sectional study with 50 neonates involved in the study. The neonates were classified into 2 groups: Case Group (25 neonates) with a diagnosis of early-onset neonatal sepsis and control group (25 neonates) without clinical manifestations of sepsis. Examination of all neonates using Transcranial Doppler (TCD) was performed within seventy-two hours after birth. Measurement of cerebral blood flow velocity (CBFV) in two major cerebral vessels: The anterior cerebral artery (ACA) and middle cerebral artery (MCA) with documentation of doppler data: The peak systolic velocity (PSV), pulsatility index (PI), and resistive index (RI). Data were statistically analyzed followed by calculation of specificity, sensitivity, positive and negative predictive value at selected cut-off values of CBFV parameters. Results: Sepsis group showed statistically significant changes in doppler indices with increased PSV and deceased PI and RI in both arteries. Conclusion:The present study demonstrates changes in CBFV measured by transcranial Doppler in newborns with EONS which can be used as noninvasive bedside investigation.