Heliocobacter pylori besides being the cause of chronic gastritis it was shown be implicated in various extra gastric manifestation. Gastric juice infected with H. pylori might have important role in upper respiratory tract. However it has not been elucidated. Therefore this study aim to clarify whether H. pylori play a role in the etiology and pathogenesis of throat infection. 307 volunteers were participated in this study. Throat swabs from tonsils and oropharynx were collected, conventional bacteriological methods were applied for identification of pathogenic bacteria, followed by PCR confirmation. Along with, serum sample were collected for determination of anti-H. pylori IgG titer by quantitative ELISA method. Prior ICT antibody-based screening test were performed. Simultaneously questionnaire data form from our participants were collected for statistical analysis. This study was designed as case control study. From throat swabs Streptococcus pyogenes was the most common in our isolates. The mean of IgG antibody titer was 95.21 RU/ml, the positive samples for IgG was quite 88.2%. Clinical respiratory symptoms were observed only in 9.7% of participants in this study 89% of them confirmed with H. pylori. Statistical data showed very high significant correlation between pathogenic bacteria S. pyogenes and H. pylori infection.