Purpose: This study was done to evaluate the effect of mandibular stem cells (MMSCs) on the rate of canine retraction. Materials and methods: The participants were twelve female patients with a bimaxillary dentoalveolar protrusion and age range from 15-21 (mean±SD 18.2 ±1.7). Bone marrow stem cells (BMSCs) were injected to one side, either right or left, while the other side served as a control group. Both upper and lower canines were retracted. Canines retraction were done under 150 gm of force using a 9 mm nickel-titanium TAD closed-coil spring between miniscrew and power arm welded to canines' bracket. Alginate impressions were taken every month until all canines had reached the second premolars, and study casts were fabricated and then scanned with a 3D scanner. Results: stem cells significantly increased the rate of tooth movement, particularly at T1. Anchorage loss was negligible and insignificant between the two groups. The pain was lower in the stem cells group, and patients were almost satisfied with the procedure. Conclusion: Non-cultured stem cell injection is a safe procedure that could be an effective method to accelerate tooth movement and significantly reduce the duration of orthodontic treatment.