Aim The purpose of this systematic review was to provide an overview of recent advances in applications of Tissue Nano-transfection and to assess its applicability in the treatment of various diseases. Materials and methods A systematic literature search was conducted in 2 electronic databases (PubMed and Science direct). The research findings were incorporated from inception. Initial screening was performed to select articles for review based on title and abstract. The full texts of selected articles were then evaluated, and relevant articles were chosen to be included in this review. Results 29 articles were identified during the literature search. There are 16 From Science Direct and 13 in PubMed. This review included 8 research articles. Conclusion TNT advancement has made a positive impact on the future of treatment of certain diseases by providing one-touch and in-site treatments. There is a need to scale up TNT experiments on animals and humans, as well as testing this approach on a broader range of diseases. Although tissue Nano-transfection technology is still in its early stages, it has already proven to be a highly promising approach in the field of regenerative medicine.

Background: Next-generation sequencing is a type of deep sequencing. In comparison to the previously used Sanger's method, Next generation sequencing allowing the sequencing of an entire genome in a single day. Next-generation sequencing (NGS) has revolutionized genomics and molecular biology. NGS has a wide range of medical applications, including tumors and inherited disease diagnosis. It is also used to find genetic variants across the genome. There are several NGS platforms available. Illumina and Iontorrent are the most prevalent sequencing platforms. These NGS platforms reduced the cost and time required to sequence a full genome. The main body of the abstract: The review paper covered a brief history of next-generation sequencing technology (NGS), followed by the benefits of employing this revolutionary technology and The general approach of NGS, beginning with fragmentation and ending with data analysis, was explained, with a focus on the Illumina and Ion torrent platforms. Finally, the data analysis step was thoroughly covered, beginning with data quality control and ending with data visualization. Conclusion: According to the review article, Next generation sequencing (NGS) is a promising technology that has revolutionized genome sequencing. The NGS platform has resulted in softwares that can perform the vast majority of NGS steps such as sequencing, variant annotation, and quality checks. The Iontorrent and Illumina platforms have grown in popularity and are frequently used. NGS has gained traction in clinical applications. Conclusion: According to the review article, Next generation sequencing (NGS) is a promising technology that has revolutionized genome sequencing. The NGS platform has resulted in softwares that can perform the vast majority of NGS steps such as sequencing, variant annotation, and quality checks. The Iontorrent and Illumina platforms have grown in popularity and are frequently used. NGS has gained traction in clinical applications.