Biological control represents an important approach for controlling many dermatophyte fungi. Pleurotuseryngiiisa promising and effective bioagent against many pathogenic fungi. In this paper Pleurotuseryngiiwere screened for their efficacy against Trichophytonrubrum. The results had been revealed that the fruiting bodies extract of the bioagentPleurotuseryngiiaffected the radial growth of the dermatophyte fungus Trichophytonrubrum. The extract of test fungus at all test concentrations had inhibitory effect on the radial growth of Trichophytonrubrum. The results had been revealed also that ethanolic extract was more effective than the aqueous extract of Pleurotuseryngiibut lower than the affectivity of the antifungal drug clotrimazole.