Hepatitis B virus (HBV) infection is a worldwide healthcare problem, especially in developing areas and the risk of liver cirrhosis in the individuals with chronic hepatitis B The purpose of the current study was to evaluate the alterations in Vitamin D3, Calcium (Ca+2) levels and Blood clotting tests [Prothrombin time (PT), Partial Thromboplastin Time (PTT), international normalized ratio (INR)], and their correlation with HBV viral load (HBV-DNA load). Vitamin D3 and Ca+2 levels and Blood clotting tests were determined in 102 patients with HBV, and 60 healthy subjects, and comparing HBV-DNA loads with the rest of the biochemical parameters. Vitamin D3 in the blood showed a significant decrease in all patients as compared to the control group (P ≤ 0.05). the results indicate that there is no significant decrease in the concentration of serum Ca+2 in patients as compared to control group, While the blood clotting tests showed a significant increase in all patients as compared to control group ( P ≤ 0.05 )The presents study showed that the correlation between HBV-DNA load and the biochemical parameters. Our findings showed a negative correlation between HBV-DNA load and (vitamin D3, Ca+2), and a positive correlation between HBV-DNA load and blood clotting tests.