This research presents the experimental and analytical results for reinforced concrete (RC) beams with rectangular large web openings under pure torsion. Fifteen specimens were tested; one solid specimen without opening, six specimens without reinforcement around opening as if an opening is created in an existing beam; where the investigated parameters were opening width, opening height and opening eccentricity from the longitudinal beam axis, and eight specimens with reinforcement installed around the opening to reduce the effect of the opening on the torsional behavior; where the investigated parameters were the shapes and amounts of the reinforcement around the opening. The test results showed that installing stirrups and horizontal reinforcement bars in the chords above and below the openings efficiently increased the cracking and ultimate torque of the tested specimens; where using inclined stirrups by 45º to the longitudinal axis of the specimens increased the ultimate torque to be about 90% of that of the solid specimen. Installing closed stirrups around the opening had small effect on enhancing the torsional behavior of the tested specimen compared with using horizontal and vertical reinforcement bars. The analytical model based on Modified Variable Angle Truss Model (MVATM) used to obtain the torque-rotation curves for beams under pure torsion was modified to incorporate the beams with large web opening which have different arrangements of reinforcement around the opening. MATLAB program language was used to obtain the analytical torque-rotation curves for the specimens that have reinforcement around the opening which were in good agreement with the experimental results.