Aim:This study aimed to distinguish carbon nanotube (CNT) particles and their pathological effects on the liver of birds in areas with carbon emissions.Materials and Methods:Twenty-one domestic ducks were collected from pure farmers and exposed to different sources of air pollution. Histological stains were used to detect the accumulation of carbon particles. In addition, acridine orange/ethidium bromide staining was used to detect apoptosis, and scanning electron microscope (SEM) technique was used to determine the morphological design of carbon particles.Results:Light microscope results showed that the liver sections contain multiwalled CNTs (MWCNTs) which appear as black spots in the hepatic parenchyma. The histopathological changes of parenchyma include sinusoidal dilatation, infiltration, and congestion with frequently high number of macrophages. In general, early destruction of hepatic parenchyma was observed. Moreover, SEM results showed two morphological types of CNTs: The ball-shaped nanoparticles scattered as ultrafine carbon black and fiber form of carbon particles were recognized as MWCNTs in the hepatic tissue. Fluorescence microscopy results showed the early and progressive stages of apoptosis in the hepatic cells of birds in polluted areas, which can be related to the degree and exposure period to pollutants.Conclusion:The study indicates that liver morbidity of birds living in the farms affected by the pollution of brick factories is higher than the birds living in farms affected by the pollution of oil fields.
The study designed to investigate the histological modification of heart tube during the development stage in local domestic rabbits embryo. The experimental animals were distributed into four groups each group consist of one male Rabbit and three female Rabbits. The pregnant female in all groups were sacrificed at the end of each period. The result showed the early stage of heart development at (4, 6, 8 and 10) days of gestation period, the first embryonic evidence of embryo was the neural tube formation belong the longitudinal axes of embryo. The result noted the primary divisions of neural tube in cephalic region of embryo. The result noted at six day of development time showed prominent cardiogenic plate located in limited region called intra embryonic cavity. The micrograph at eight day of heart development showed the heart tube have prominent wide ends, while at ten day noted establishment of primitive heart regions which included the truncus areriosus, bulbo cordis, atrium, ventricle and sinus venosus .
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