The blue-green algae (Spirulina platensis) is widely distributed worldwide. The nutritional value of Spirulina algae is well-documented. Spirulina has unique high protein content, about 50-70% by dry weight, and it is considered as a good source of essential amino acid. Additionally, Spirulina was recognised to have a wide range of essential nutrients, including essential fatty acids and polysaccharides, vitamins and minerals, and carotenoids.The aim of this study was to determine the injection effect of spirulina liquid extract on fertility and hatching traits, production performance, and some biochemical characteristics of quail eggs during the incubation period. Four hundred and fifty eggs were selected for hatching. The eggs were divided into three groups, and every group was treated differently. The results of the first treatment showed that there was a significant difference in the hatchability percentage of fertilized eggs. Furthermore, the percentage of failed eggs and weak chicks from the control treatment is almost significantly. The results of the second treatment showed that there was no effect on the average weight of the hatched chicks and feed conversion ratio. The third treatment showed a significant increase in the weight gain and feed consumed. It was noticed that the treatments did not have a notable effect on the relative weight of the liver, heart, and intestines. No significant differences were observed of physiological characteristics, as total protein concentration, enzymatic activity of GOT, GPT, and MDA level. The third treatment caused an increase in GSH glutathione level comparison with a control treatment. The injection of spirulina liquid extracts in late stages of incubation could improve the hatchability percentage chicks and their chances of survival. It can also strengthen the new hatch chicks immunity and antioxidants status.