investigation of Date Palm pollen revealed the presence of sterols, triterpenes, saponins, proteins, carbohydrates andlor glycosides and absence of volatile substances. From the U.S.M., B-amyrin, 8-sitosterol and a third crystalline substance were isolated. Four flavonoids were detected by TLC; two.of them were identified as rutin and quercetin. A steroidal saponin glycoside was isolated, having glucose and rhamnose as sugar moiety. A glucoprotein was isolated and proved biologically to have a gonadotrophic activity. TLC analysisprovedpresence of oesterone: Introdtrction Date Palm (Phoenix dactylifera L.; Palmae) is widely growing in Egypt and the pollen grains have been held for generations to be nutritive and therapeutic as an antisterility agent. ABOU EL-WAFA [I] reported the presence of a non-crystalline oestrogenic substance in the ether extract of-Date pollen; besides rutin, a saponin-like substance and sucrose. EL-RIDI [2] isolated, in a crude state, a gonadotrophicallyactive substance from the pollen, which was found to be toxic when injected into rats. B E N E~E [3] isolated oesterone and cholesterol from Date Palm pollen in U.S.A. FODA [4] reported the presence of a growth hormone in the alcohol extract of Date Palm pollen. In view of the significance of pollen grains a t the present time, we decided to reinvestigate its constituents applying some recent methods of identification, isolation and analysis. Materials The air-dried pollen grains of Date palm were collected during March and April 1972, 1973 and 1974 from Date palms growing in the Delta region of Egypt, mainly from Sharkia Province and immediately used after collection.
Background: Lantana montevidensis (L. montevidensis) is native to Brazil, the tea and infusions of the dried leaves have been used in folk medicine. Previous study of L. montevidensis leaves has resulted in the isolation of ten anti-proliferative fl avones. Also, the essential oil of the Brazilian L. montevidensis leaves was investigated for its chemical composition and antimicrobial activity. Furthermore, the ethanolic extracts of the leaves and roots demonstrated anti-bacterial activity. Materials and Methods: The essential oils extracted by hydrodistillation from the Egyptian L. montevidensis leaves and fl owers were investigated for their chemical composition. Also, a qualitative phytochemical analysis of 70% aqueous methanolic extract of L. montevidensis leaves was performed for the detection of alkaloids, tannins, triterpenoids, steroids, fl avonoids, anthraquinones, saponins, iridoids, carbohydrates and/or glycosides. Furthermore, the extracts from the leaves were assessed for their anti-infl ammatory, anti-pyretic, analgesic, antioxidant and antibacterial activities. Results: The composition of the essential oils included large amount of sesquiterpenes, mainly caryophyllene (33.74%), ß-selinene (43.95%), germacrene-D (3.79%) and nerolidol (6.32%) in the oil of the leaves, while caryophyllene (42.38%), γ-terpinene (22.64%), D-germacrene (9.10 %) and nerolidol (7.09%) are major in the oil of the fl owers. Phytochemical screening of 70% aqueous methanolic extract of L. montevidensis leaves revealed the presence of carbohydrates and/or glycosides, unsaturated sterols, triterpenoids and fl avonoids in addition to traces of saponins and iridoids. Furthermore, the extracts from the leaves exhibited anti-infl ammatory, anti-pyretic, analgesic, antioxidant and antibacterial activities. Conclusion: The obtained results may provide a support to explore the plant for isolation of the active constituents accountable for these activities.
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