Nanobiosensors are convenient, practical, and sensitive analyzers that detect chemical and biological agents and convert the results into meaningful data between a biologically active molecule and a recognition element immobilized on the surface of the signal transducer by a physicochemical detector. Due to their fast, accurate and reliable operating characteristics, nanobiosensors are widely used in clinical and nonclinical applications, bedside testing, medical textile industry, environmental monitoring, food safety, etc. They play an important role in such critical applications. Therefore, the design of the biosensing interface is essential in determining the performance of the nanobiosensor. The unique chemical and physical properties of nanomaterials have paved the way for new and improved sensing devices in biosensors. The growing demand for devices with improved sensing and selectivity capability, short response time, lower limit of detection, and low cost causes novel investigations on nanobiomaterials to be used as biosensor scaffolds. Among all other nanomaterials, studies on developing nanobiosensors based on metal oxide nanostructures, graphene and its derivatives, carbon nanotubes, and the widespread use of these nanomaterials as a hybrid structure have recently attracted attention. Nanohybrid structures created by combining these nanostructures will directly meet the future biosensors’ needs with their high electrocatalytic activities. This review addressed the recent developments on these nanomaterials and their derivatives, and their use as biosensor scaffolds. We reviewed these popular nanomaterials by evaluating them with comparative studies, tables, and charts.

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